ActinoBase: tools and protocols for researchers working on Streptomyces and other filamentous actinobacteria

Feeney, M. A. et al. (2022) ActinoBase: tools and protocols for researchers working on Streptomyces and other filamentous actinobacteria. Microbial Genomics, 8(7), 000824. (doi: 10.1099/mgen.0.000824) (PMID:35775972) (PMCID:PMC9455695)

Abstract

Actinobacteria is an ancient phylum of Gram-positive bacteria with a characteristic high GC content to their DNA. The ActinoBase Wiki is focused on the filamentous actinobacteria, such as Streptomyces species, and the techniques and growth conditions used to study them. These organisms are studied because of their complex developmental life cycles and diverse specialised metabolism which produces many of the antibiotics currently used in the clinic. ActinoBase is a community effort that provides valuable and freely accessible resources, including protocols and practical information about filamentous actinobacteria. It is aimed at enabling knowledge exchange between members of the international research community working with these fascinating bacteria. ActinoBase is an anchor platform that underpins worldwide efforts to understand the ecology, biology and metabolic potential of these organisms. There are two key differences that set ActinoBase apart from other Wiki-based platforms: [1] ActinoBase is specifically aimed at researchers working on filamentous actinobacteria and is tailored to help users overcome challenges working with these bacteria and [2] it provides a freely accessible resource with global networking opportunities for researchers with a broad range of experience in this field.

• Item Type: Articles
• Additional Information: LKC is supported by a National Science Foundation CAREER Award (CHE-1652424). PAH would like to acknowledge funding from NERC (NE/M001415), iUK (BB/N023544/1), BBSRC (BB/T001038/1; BB/R022054/1; BB/T004126/1; BB/N023544/1; BB/V000993/1; BB/V017586/1), The Royal Society Newton Fund (NAF\R2\180631), The Industrial Biotechnology Innovation Centre (for studentship support to ASB, MK-B), The University of Strathclyde (for studentship support to REM and EA) the Scottish University Life Science Alliance (for studentship support to JKS) and the Royal Academy of Engineering Research Chair Scheme for long term personal research support (RCSRF2021\11\15). HO was supported by the US Department of Energy Office of Science under Contract No. DE-AC02-05CH11231. KV has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 765 147. RFS is supported by BBSRC responsive mode grants BB/T008075/1 and BB/T014962/1. KRD’s laboratory has received funding from a Carnegie Trust Collaborative Research Grant and the Biotechnology and Biological Sciences Research Council (BBSRC; BB/R022054/1). Research in KRD’s laboratory has been carried out by author JP and others. JP was supported by a studentship from the Programa de Innovación y Capital Humano para la Competitividad (PINN) of The Ministry of Science, Technology and Telecommunications of Costa Rica (MICITT), grant 2-1-4-17-1-037. Research in LTFM’s laboratory has been carried out by JKS and supported by BBSRC responsive mode grant BB/S016651/1. Research in MIH’s laboratory has been carried out by authors LB, RD, DG, JH, TCM, JTN, KN, LP, SMMP, SFW and others and has been supported by responsive mode funding from the BBSRC (BB/W000628, BB/T015446/1, BB/S00811X/1, BB/P005292/1 and BB/F009429/1 and Institute Strategic Programme Project BBS/E/J/000PR9790 to the JIC), the Medical Research Council (G0801721) and the Natural Environment Research Council (NE/M015033/1 and NE/J01074X/1). Additional funding has been provided by the Royal Society, the Norwich Research Park, the University of East Anglia, the John Innes Centre, BBSRC Pathfinder and Follow-on funding and via BBSRC- and NERC-funded PhD studentships (BBSRC doctoral training programme grant BB/M011216/1 and NERC doctoral training programme grant NE/L002582/1).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mark, Dr David
• Authors: Feeney, M. A., Newitt, J. T., Addington, E., Algora-Gallardo, L., Allan, C., Balis, L., Birke, A. S., Castaño-Espriu, L., Charkoudian, L. K., Devine, R., Gayrard, D., Hamilton, J., Hennrich, O., Hoskisson, P. A., Keith-Baker, M., Klein, J. G., Kruasuwan, W., Mark, D. R., Mast, Y., McHugh, R. E., McLean, T. C., Mohit, E., Munnoch, J. T., Murray, J., Noble, K., Otani, H., Parra, J., Pereira, C. F., Perry, L., Pintor-Escobar, L., Pritchard, L., Prudence, S. M. M., Russell, A. H., Schniete, J. K., Seipke, R. F., Sélem-Mojica, N., Undabarrena, A., Vind, K., van Wezel, G. P., Wilkinson, B., Worsley, S. F., Duncan, K. R., Fernández-Martínez, L. T., and Hutchings, M. I.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Microbial Genomics
• Publisher: Microbiology Society
• ISSN: 2057-5858
• ISSN (Online): 2057-5858
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in Microbial Genomics 8:000824
• Publisher Policy: Reproduced under a creative commons licence