Adaptation to endophytic lifestyle through genome reduction by Kitasatospora sp. SUK42

Zin, N. M., Ismail, A., Mark, D. R. , Westrop, G., Schniete, J. K. and Herron, P. R. (2021) Adaptation to endophytic lifestyle through genome reduction by Kitasatospora sp. SUK42. Frontiers in Bioengineering and Biotechnology, 9, 740722. (doi: 10.3389/fbioe.2021.740722) (PMID:34712653) (PMCID:PMC8545861)

Abstract

Endophytic actinobacteria offer great potential as a source of novel bioactive compounds. In order to investigate the potential for the production of secondary metabolites by endophytes, we recovered a filamentous microorgansism from the tree Antidesma neurocarpum Miq. After phenotypic analysis and whole genome sequencing we demonstrated that this organism, SUK42 was a member of the actinobacterial genus Kitasatospora. This strain has a small genome in comparison with other type strains of this genus and has lost metabolic pathways associated with Stress Response, Nitrogen Metabolism and Secondary Metabolism. Despite this SUK42 can grow well in a laboratory environment and encodes a core genome that is consistent with other members of the genus. Finally, in contrast to other members of Kitasatospora, SUK42 encodes saccharide secondary metabolite biosynthetic gene clusters, one of which with similarity to the acarviostatin cluster, the product of which displays α-amylase inhibitory activity. As extracts of the host plant demonstrate this inhibitory activity, it suggests that the potential medicinal properties of A. neurocarpum Miq might be provided by the endophytic partner and illustrate the potential for exploitation of endophytes for clinical or industrial uses.

• Item Type: Articles
• Additional Information: This work received financial support from the Ministry of Higher Education (Grant number ERGS/1/2013/SKK04/UKM/02/2) and the Centre for Research and Innovation Management (CRIM), Universiti Kebangsaan Malaysia (NMZ and AI). DRM was supported by a University of Strathclyde and a Tools and Resources Development Fund award from the Biotechnology and Biological Sciences Research Council (grant number BB/ M018792/1) (JKS) was awarded to PRH.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Westrop, Mr Gareth and Mark, Dr David
• Authors: Zin, N. M., Ismail, A., Mark, D. R., Westrop, G., Schniete, J. K., and Herron, P. R.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Frontiers in Bioengineering and Biotechnology
• Publisher: Frontiers Media SA
• ISSN: 2296-4185
• ISSN (Online): 2296-4185
• Copyright Holders: Copyright ©2021 Zin, Ismail, Mark, Westrop, Schniete and Herron.
• First Published: First published in Frontiers in Bioengineering and Biotechnology 9:740722
• Publisher Policy: Reproduced under a creative commons licence