Bilateral symmetry of linear streptomycete chromosomes

Algora-Gallardo, L., Schniete, J. K., Mark, D. R. , Hunter, I. S. and Herron, P. R. (2021) Bilateral symmetry of linear streptomycete chromosomes. Microbial Genomics, 7(11), 000692. (doi: 10.1099/mgen.0.000692) (PMID:34779763) (PMCID:PMC8743542)

Abstract

Here, we characterize an uncommon set of telomeres from Streptomyces rimosus ATCC 10970, the parental strain of a lineage of one of the earliest-discovered antibiotic producers. Following the closure of its genome sequence, we compared unusual telomeres from this organism with the other five classes of replicon ends found amongst streptomycetes. Closed replicons of streptomycete chromosomes were organized with respect to their phylogeny and physical orientation, which demonstrated that different telomeres were not associated with particular clades and are likely shared amongst different strains by plasmid-driven horizontal gene transfer. Furthermore, we identified a ~50 kb origin island with conserved synteny that is located at the core of all streptomycete chromosomes and forms an axis around which symmetrical chromosome inversions can take place. Despite this chromosomal bilateral symmetry, a bias in parS sites to the right of oriC is maintained across the family Streptomycetaceae and suggests that the formation of ParB/parS nucleoprotein complexes on the right replichore is a conserved feature in streptomycetes. Consequently, our studies reveal novel features of linear bacterial replicons that, through their manipulation, may lead to improvements in growth and productivity of this important industrial group of bacteria.

• Item Type: Articles
• Additional Information: This work was supported by studentships awarded by the Industrial Biotechnology Innovation Centre (L.A.-G.) and the University of Strathclyde (D.R.M.), as well as a Tools and Resources Development Fund award from the Biotechnology and Biological Sciences Research Council (grant number BB/M018792/1) (J.K.S.) awarded to P.R.H.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mark, Dr David and Algora Gallardo, Miss Lis
• Authors: Algora-Gallardo, L., Schniete, J. K., Mark, D. R., Hunter, I. S., and Herron, P. R.
• College/School: College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Microbial Genomics
• Publisher: Microbiology Society
• ISSN: 2057-5858
• ISSN (Online): 2057-5858
• Published Online: 15 November 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Microbial Genomics 7:000692
• Publisher Policy: Reproduced under a creative commons licence