Urinary peptidomics and pulse wave velocity: The African-PREDICT study

de Beer, D., MC. Mels, C., Schutte, A., Delles, C. , Mary, S. , Mullen, W. , Mischak, H. and Kruger, R. (2023) Urinary peptidomics and pulse wave velocity: The African-PREDICT study. Journal of Proteome Research, 22(10), pp. 3282-3289. (doi: 10.1021/acs.jproteome.3c00347) (PMID:37688558) (PMCID:PMC10563154)

Abstract

Increased arterial stiffness is related to early vascular aging and is an independent predictor for cardiovascular disease and mortality. Molecular mechanisms underlying increased arterial stiffness are largely unexplored, especially at the proteome level. We aimed to explore the relationship between pulse wave velocity and urinary proteomics. We included 919 apparently healthy (no chronic illnesses) Black and White men and women (equally distributed) between 20 and 30 years from the African-PREDICT study. Capillary electrophoresis time-of-flight mass spectrometry was used to analyze the urinary proteome. We measured the carotid-femoral pulse wave velocity to estimate arterial stiffness. In the total group, pulse wave velocity correlated positively with collagen-derived peptides including collagen types I, II, III, IV, V, and IX and inversely with collagen type XI (adjusted for mean arterial pressure). Regarding noncollagen-derived peptides, pulse wave velocity positively correlated with polymeric immunoglobulin receptor peptides (n = 2) (all q-value ≤0.05). In multivariable adjusted analyses, pulse wave velocity associated positively and independently with seven urinary peptides (collagen type I, n = 5) (all p-value ≤0.05). We found significant positive and independent associations between pulse wave velocity and the collagen type I-derived peptides, suggesting that dysregulation of collagen type I in the extracellular matrix scaffold could lead to early onset of increased arterial stiffness.

• Item Type: Articles
• Additional Information: The research funded in this manuscript is part of an ongoing research project financially supported by the South African Medical Research Council (SAMRC) with funds from National Treasury under its Economic Competitiveness and Support Package; the South African Research Chairs Initiative (SARChI) of the Department of Science and Technology and National Research Foundation (NRF) of South Africa (GUN86895); SAMRC with funds received from the South African National Department of Health, GlaxoSmith Kline R and D (Africa Non-Communicable Disease Open Lab grant), the UK Medical Research Council and with funds from the UK Government’s Newton Fund; as well as corporate social investment grants from Pfizer (South Africa), Boehringer-Ingelheim (South Africa), Novartis (South Africa), the MediClinic Hospital Group (South Africa) and in kind contribu-tions of Roche Diagnostics (South Africa)
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mullen, Dr Bill and Samji, Dr Sheon and Delles, Professor Christian
• Authors: de Beer, D., MC. Mels, C., Schutte, A., Delles, C., Mary, S., Mullen, W., Mischak, H., and Kruger, R.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Journal of Proteome Research
• Publisher: American Chemical Society
• ISSN: 1535-3893
• ISSN (Online): 1535-3907
• Copyright Holders: Copyright: © 2023 The Authors
• First Published: First published in Journal of Proteome Research 22(10): 3282–3289
• Publisher Policy: Reproduced under a Creative Commons licence