Ovciarikova, J. et al. (2024) Two ancient membrane pores mediate mitochondrial-nucleus membrane contact sites. Journal of Cell Biology, 223(4), e202304075. (doi: 10.1083/jcb.202304075) (PMID:38456969)
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Abstract
Coordination between nucleus and mitochondria is essential for cell survival, and thus numerous communication routes have been established between these two organelles over eukaryotic cell evolution. One route for organelle communication is via membrane contact sites, functional appositions formed by molecular tethers. We describe a novel nuclear-mitochondrial membrane contact site in the protozoan Toxoplasma gondii. We have identified specific contacts occurring at the nuclear pore and demonstrated an interaction between components of the nuclear pore and the mitochondrial protein translocon, highlighting them as molecular tethers. Genetic disruption of the nuclear pore or the TOM translocon components, TgNup503 or TgTom40, respectively, result in contact site reduction, supporting their potential involvement in this tether. TgNup503 depletion further leads to specific mitochondrial morphology and functional defects, supporting a role for nuclear-mitochondrial contacts in mediating their communication. The discovery of a contact formed through interaction between two ancient mitochondrial and nuclear complexes sets the ground for better understanding of mitochondrial-nuclear crosstalk in eukaryotes.
| Item Type: | Articles |
|---|---|
| Additional Information: | Funders: Wellcome Trust, grant 217173/Z/19/Z; and the Biotechnology and Biological Sciences Research Council, grant BB/N003675/1 to L. Sheiner; French National Research Agency, grant number ANR-13-JSV3-0006-01 to M. Gissot. E.S. Martins-Duarte received support from Pró-Reitoria de Pesquisa-UFMG and Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (408964/2018-9). S. Shikha is funded by the Swiss National Foundation Early Postdoc Mobility fellowship. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ovciarikova, Miss Jana and Lacombe, Alice and MacLean, Dr Andrew and Hussain, Dr Wasim and Sheiner, Professor Lilach and Lemgruber Soares, Dr Leandro |
| Creator Roles: | Ovciarikova, J.Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – review and editing Lacombe, A.Investigation Hussain, W.Investigation MacLean, A.Investigation Lemgruber, L.Formal analysis, Investigation, Methodology, Visualization Sheiner, L.Conceptualization, Funding acquisition, Project administration, Supervision, Investigation, Writing – original draft, Writing – review and editing |
| Authors: | Ovciarikova, J., Shikha, S., Lacombe, A., Courjol, F., McCrone, R., Hussain, W., MacLean, A., Lemgruber, L., Martins-Duarte, E. S., Gissot, M., and Sheiner, L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Cell Biology |
| Publisher: | Rockefeller University Press |
| ISSN: | 0021-9525 |
| ISSN (Online): | 1540-8140 |
| Published Online: | 08 March 2024 |
| Copyright Holders: | Copyright © 2024 Ovciarikova et al. |
| First Published: | First published in Journal of Cell Biology 223(4):e202304075 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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