Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing

Shlyakhtina, Y., Bloechl, B., Moran, K. l. and Portal, M. M. (2024) Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing. STAR Protocols, 5(1), 102809. (doi: 10.1016/j.xpro.2023.102809) (PMID:38180835) (PMCID:PMC10801334)

Abstract

Here, we present a protocol to perform barcode decay lineage tracing followed by single-cell transcriptome analysis (BdLT-Seq). We describe steps for BdLT-Seq experimental design, building barcoded episome reporters, performing episome transfection, and barcode retrieval. We then describe procedures for sequencing library construction while providing options for sample multiplexing and data analysis. This BdLT-Seq technique enables the assessment of clonal evolution in a directional manner while preserving isogeneity, thus allowing the comparison of non-genetic molecular features between isogenic cell lineages. For complete details on the use and execution of this protocol, please refer to Shlyakhtina et al. (2023). . [Abstract copyright: Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.]

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Shlyakhtina, Miss Yelyzaveta and Portal, Dr Maximiliano
• Authors: Shlyakhtina, Y., Bloechl, B., Moran, K. l., and Portal, M. M.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: STAR Protocols
• Publisher: Cell Press
• ISSN: 2666-1667
• ISSN (Online): 2666-1667
• Copyright Holders: Copyright © 2023 The Author(s)
• First Published: First published in STAR Protocols 5(1):102809
• Publisher Policy: Reproduced under a creative commons licence