Castejón-Vega, B. , Kyriakidis, N. C., Alcócer-Gómez, E., Giampieri, F., González-Paramás, A. M., Cordero, M. D. and Alvarez-Suarez, J. M. (2023) Modulatory effect of Andean blackberry polyphenols on genes related to antioxidant and inflammatory responses, the NLRP3 inflammasome, and autophagy. Journal of Berry Research, (doi: 10.3233/jbr-230058) (Early Online Publication)
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Abstract
BACKGROUND: The Andean blackberry (Rubus glaucus Benth) is one of Ecuador’s most iconic Andean berries for which a high anthocyanin content has been described. OBJECTIVE: The aim of the present study was to determine the chemical composition and anti-inflammatory potential of the Andean blackberry from Ecuador, with an emphasis on its effects on NLRP3 inflammasome activation and autophagy processes. RESULTS: Andean blackberry extracts were rich in hydroxycinnamates (coumaric acid and derivates), in addition to quercetin and kaempferol as principal flavonols. Cyanidin and its glycosides were identified as the main anthocyanins present. Andean blackberry extracts efficiently reduced oxidative stress markers in the lipopolysaccharide-stimulated RAW 264.7 cells. The extracts also caused a moderate decrease in the expression of the pro-inflammatory and antioxidant genes NFκB1, TNF, IL-1β, IL-6, and NOS2 expression, while they significantly increased the mRNA levels of both SOD1 and NFE2L2 genes. Andean blackberry extracts significantly decreased the activation of the NLRP3 inflammasome complex, as well as p62 levels, and the LC3I/LC3II ratio increased, suggesting a direct action of Andean blackberry compounds on the inflammatory response and restoration of the autophagy process. CONCLUSIONS: These results suggest that Andean blackberries potentially have an anti-inflammatory effect through their ability to regulate genes related to the inflammatory and antioxidant response, as well as modulate the activation of the NLRP3 inflammasome complex and autophagy processes.
Item Type: | Articles |
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Keywords: | Horticulture, Plant Science, Soil Science, Agronomy and Crop Science, Biochemistry, Food Science |
Status: | Early Online Publication |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Castejon Vega, Dr Beatriz |
Authors: | Castejón-Vega, B., Kyriakidis, N. C., Alcócer-Gómez, E., Giampieri, F., González-Paramás, A. M., Cordero, M. D., and Alvarez-Suarez, J. M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Journal of Berry Research |
Publisher: | IOS Press |
ISSN: | 1878-5093 |
ISSN (Online): | 1878-5123 |
Published Online: | 30 December 2023 |
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