Freeman, M., Huethorst, E. , Boland, E., Dunne, M., Burton, F., Denning, C., Myles, R. and Smith, G. (2024) A novel method for the percutaneous induction of myocardial infarction by occlusion of small coronary arteries in the rabbit. American Journal of Physiology: Heart and Circulatory Physiology, (doi: 10.1152/ajpheart.00657.2023) (PMID:38180449) (Early Online Publication)
![[img]](https://eprints.gla.ac.uk/style/images/fileicons/text.png) |
Text
316218.pdf - Accepted Version Restricted to Repository staff only until 5 January 2025. 1MB |
Abstract
Arrhythmic sudden cardiac death (SCD) is an important cause of mortality following myocardial infarction (MI). The rabbit has similar cardiac electrophysiology to humans and is therefore an important small animal model to study post-MI arrhythmias. The established approach of surgical coronary ligation results in thoracic adhesions which impede epicardial electrophysiological studies. Adhesions are absent following a percutaneously induced MI, which is also associated with reduced surgical morbidity and so represents a clear refinement of the approach. Percutaneous procedures have previously been described in large rabbits (3.5-5.5kg). Here we describe a novel method of percutaneous MI induction in the smaller rabbits (2.5-3.5kg) that are readily available commercially. New Zealand White rabbits (n=51 male, 3.1±0.3 kg) were anesthetized using isoflurane (1.5-3%) and underwent either a percutaneous MI procedure involving microcatheter tip deployment (≤1.5F, 5mm), coronary ligation surgery or a sham procedure. ECG recordings were used to confirm ST-segment elevation indicating coronary occlusion. Blood samples (1h and 24h) were taken for cardiac troponin I (cTnI) levels. Ejection fraction (EF) was measured at 6-8 weeks. Rabbits were then euthanized (Euthatal) and hearts processed for magnetic resonance imaging and histology. Mortality rates were similar in both groups. Scar volume, cTnI and EF were similar between both MI groups and significantly different from their respective sham controls. Thus, percutaneous coronary occlusion by microcatheter tip deployment is feasible in rabbits (2.5-3.5kg) and produces an MI with similar characteristics to surgical ligation with lower procedural trauma and without epicardial adhesions.
| Item Type: | Articles |
|---|---|
| Additional Information: | EH, CD, RM, CD and GS were funded by the British Heart Foundation Centre for Regenerative Medicine with grant number CRMR/21/290009. MF, EH, RM and GS were funded by the BHF Centre of Research Excellence with Grant Number RE/18/63/42/17. EB, RM, MD and GS were funded by the BHF project grant number PG/19/55/34545. CD was funded by BHF project grant number PG/21/10545. EH was awarded The Physiological Society Conference Attendance Award with grant number 41317-FR. |
| Status: | Early Online Publication |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Burton, Dr Francis and Freeman, Dr Michael and Myles, Dr Rachel and Dunne, Mr Michael and Smith, Professor Godfrey and Boland, Dr Erin and Huethorst, Dr Eline |
| Authors: | Freeman, M., Huethorst, E., Boland, E., Dunne, M., Burton, F., Denning, C., Myles, R., and Smith, G. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | American Journal of Physiology: Heart and Circulatory Physiology |
| Publisher: | American Physiological Society |
| ISSN: | 0363-6135 |
| ISSN (Online): | 1522-1539 |
| Published Online: | 05 January 2024 |
| Copyright Holders: | Copyright: © 2024, American Journal of Physiology-Heart and Circulatory Physiology |
| First Published: | First published in American Journal of Physiology: Heart and Circulatory Physiology 2024 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information