Aqueel, R., Badar, A., Roy, N., Mushtaq, Q., Ali, A. F., Bashir, A., Ijaz, U. Z. and Malik, K. A. (2023) Cotton microbiome profiling and Cotton Leaf Curl Disease (CLCuD) suppression through microbial consortia associated. npj Biofilms and Microbiomes, 9(1), 100. (doi: 10.1038/s41522-023-00470-9) (PMID:38097579) (PMCID:PMC10721634)
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Abstract
The failure of breeding strategies has caused scientists to shift to other means where the new approach involves exploring the microbiome to modulate plant defense mechanisms against Cotton Leaf Curl Disease (CLCuD). The cotton microbiome of CLCuD-resistant varieties may harbor a multitude of bacterial genera that significantly contribute to disease resistance and provide information on metabolic pathways that differ between the susceptible and resistant varieties. The current study explores the microbiome of CLCuD-susceptible Gossypium hirsutum and CLCuD-resistant Gossypium arboreum using 16 S rRNA gene amplification for the leaf endophyte, leaf epiphyte, rhizosphere, and root endophyte of the two cotton species. This revealed that Pseudomonas inhabited the rhizosphere while Bacillus was predominantly found in the phyllosphere of CLCuV-resistant G. arboreum. Using salicylic acid-producing Serratia spp. and Fictibacillus spp. isolated from CLCuD-resistant G. arboreum, and guided by our analyses, we have successfully suppressed CLCuD in the susceptible G. hirsutum through pot assays. The applied strains exhibited less than 10% CLCuD incidence as compared to control group where it was 40% at 40 days post viral inoculation. Through detailed analytics, we have successfully demonstrated that the applied microbes serve as a biocontrol agent to suppress viral disease in Cotton.
| Item Type: | Articles |
|---|---|
| Additional Information: | This project is supported by Research Linkages Grant from Alexander Von Humboldt Foundation, Germany Grant No. 3.4-1017354-Pak and Pakistan Academy of Sciences Grant No 181 awarded to KAM. UZI acknowledges support from UK Research and Innovation: Natural Environment Research Council NERC NE/L011956/1 and Engineering and Physical Science Research Council EPSRC EP/V030515/1. Some part of the work is conducted in University of Glasgow with mobility support to RA through International Research Support Initiative Program (IRSIP) Project No. 1-8/HEC/HRD/2023/12777 under Higher Education Commission, Pakistan. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Aqueel, Rhea and Ijaz, Dr Umer |
| Creator Roles: | Aqueel, R.Conceptualization, Visualization, Data curation, Investigation, Formal analysis, Writing – original draft Ijaz, U. Z.Methodology, Software, Formal analysis, Writing – original draft, Supervision, Funding acquisition |
| Authors: | Aqueel, R., Badar, A., Roy, N., Mushtaq, Q., Ali, A. F., Bashir, A., Ijaz, U. Z., and Malik, K. A. |
| College/School: | College of Science and Engineering College of Science and Engineering > School of Engineering > Infrastructure and Environment |
| Journal Name: | npj Biofilms and Microbiomes |
| Publisher: | Nature Research |
| ISSN: | 2055-5008 |
| ISSN (Online): | 2055-5008 |
| Published Online: | 14 December 2023 |
| Copyright Holders: | Copyright © The Author(s) 2023 |
| First Published: | First published in npj Biofilms and Microbiomes 9(1):100 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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