E5 protein of human papillomavirus 16 downregulates HLA class I and interacts with the heavy chain via its first hydrophobic domai

Ashrafi, G.H., Haghshenas, M., Marchetti, B. and Campo, M.S. (2006) E5 protein of human papillomavirus 16 downregulates HLA class I and interacts with the heavy chain via its first hydrophobic domai. International Journal of Cancer, 119(9), pp. 2105-2112. (doi: 10.1002/ijc.22089)

Abstract

Human papillomavirus type 16 E5 protein (HPV-16 E5) is expressed early in papillomavirus infection and is localised primarily in the cell Golgi apparatus (GA) and endoplasmic reticulum. E5 prevents transport of the major histocompatibility class I (MHC I; HLA class I in humans) to the cell surface and retains the complex in the GA. We report that these effects are due, at least in part, to the interaction between E5 and HLA I heavy chain (HC). We also demonstrate that the down-regulation of surface HLA I and interaction with HC are mediated by the first hydrophobic domain of E5. Although E5 downregulates classical HLA selectively as it does not downregulate non-classical HLA, the interaction with the HC of classical HLA I is not specific for a particular haplotype of HLA I. This suggests that E5 can interfere with antigen presentation by most, if not all, classical HLA I haplotypes, with potentially serious consequences as the ability of infected cells to present antigenic peptides to effector T cells would be compromised.

• Item Type: Articles
• Keywords: HPV, E5, HLA class I, heavy chain, interaction, immunomodulation
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ashrafi, Dr Gholam Hossein and Campo, Professor Saveria and Marchetti, Dr Barbara
• Authors: Ashrafi, G.H., Haghshenas, M., Marchetti, B., and Campo, M.S.
• Subjects: S Agriculture > SF Animal culture > SF600 Veterinary Medicine; R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer); Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: International Journal of Cancer
• Publisher: John Wiley and Sons
• ISSN: 0020-7136
• Copyright Holders: Copyright © 2006 John Wiley and Sons
• First Published: First published in International Journal of Cancer 119(9):2105-2112
• Publisher Policy: Reproduced with the permission of the publisher