A New Platform for Single Molecule Measurements Using the Fluorous Effect

Santana Vega, M. et al. (2023) A New Platform for Single Molecule Measurements Using the Fluorous Effect. In: SPIE BiOS 2023: Single Molecule Spectroscopy and Superresolution Imaging XVI, San Francisco, CA, USA, 28 Jan - 03 Feb 2023, (doi: 10.1117/12.2647953)

Abstract

Irreversible adsorption of biomolecules onto imaging substrates is an impediment to expand the applications of single molecule techniques. Traditional polyethylene glycol (PEG) surfaces are only effective at low concentrations of analytes and their structure prevents their use for interferometric scattering (iSCAT) microscopy. We propose a new platform that virtually eliminates non-specific binding thanks to the omniphobicity of perfluorinated compounds, also known as the fluorous effect. Here, we showcase the anti-fouling properties of these substrates at a single molecule level through iSCAT measurements of a protein mixture. We believe these novel engineered substrates show great promise to study biomachinery processes requiring large analyte concentrations, where other passivation methods are not effective, through iSCAT microscopy and other single molecule techniques.

• Item Type: Conference Proceedings
• Keywords: Single-molecule imaging, interferometric scattering microscopy, mass photometry, surface passivation, fluorous effect.
• Status: Published
• Refereed: No
• Glasgow Author(s) Enlighten ID: Santana Vega, Dr Marina and Clark, Professor Alasdair
• Authors: Santana Vega, M., Bueno-Alejo, C. J., Sender, A. T., Chaplin, A. K., Farrow, C., Axer, A., Burley, G. A., Dominguez, C., Kara, H., Paschalis, V., Tubasum, S., Eperon, I. C., Hudson, A. J., and Clark, A. W.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• ISSN: 1605-7422
• Copyright Holders: Copyright © 2023 SPIE
• First Published: First published in Proceedings of SPIE 12386:123860A
• Publisher Policy: Reproduced in accordance with the publisher copyright policy