Song, Y. , Yin, J., Huang, W. E., Li, B. and Yin, H. (2024) Emerging single-cell microfluidic technology for microbiology. Trends in Analytical Chemistry, 170, 117444. (doi: 10.1016/j.trac.2023.117444)
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Abstract
Cell-to-cell differences exist ubiquitously in microbial communities. This individual heterogeneity, often manifested in cell phenotypic function (e.g., antibiotic resistance), can be critical in determining the fate of a microbial community. However, studying such heterogeneity in microbial communities remains a significant challenge due to their enormous diversity and complex cell-cell interactions. Here we review recent advances in microfluidic technologies for detecting, manipulating, and sorting microbial populations at the single-cell level, which significantly advanced our understanding of microbial behaviour and their roles in the microbial ecosystem. We will highlight microfluidic systems with label-free detection methods, including optical imaging and Raman spectroscopy, due to their advantages in investigating real-world microbial communities. We will showcase these technologies in emerging applications, including rapid diagnosis of pathogens and antibiotic resistance, chemotaxis, and Raman-activated cell sorting to search for natural microbial cells of desirable phenotypic function.
| Item Type: | Articles |
|---|---|
| Additional Information: | We acknowledge the support from the EPSRC IAA (EP/X5257161/1 and EP/R511705/1) and InnovateUK (104984). WEH thank EPSRC (EP/M02833X/1) for instrumentation support, as well as finance support from EPSRC (EP/M002403/1) and international collaboration project between University of Oxford and Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences. |
| Keywords: | Single-cell microfluidics, microbial communities, Raman spectroscopy, antibiotic resistance, droplet microfluidics. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing and Song, Dr Yanqing |
| Authors: | Song, Y., Yin, J., Huang, W. E., Li, B., and Yin, H. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Trends in Analytical Chemistry |
| Publisher: | Elsevier |
| ISSN: | 0165-9936 |
| ISSN (Online): | 1879-3142 |
| Published Online: | 18 November 2023 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Trends in Analytical Chemistry 170:117444 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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