Silva-Alvim, F. A.L., Alvim, J. C., Harvey, A. and Blatt, M. R. (2023) Speedy stomata of a C4 plant correlate with enhanced K+ channel gating. Plant, Cell and Environment, (doi: 10.1111/pce.14775) (Early Online Publication)
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Abstract
Stomata are microscopic pores at the surface of plant leaves that facilitate gaseous diffusion to support photosynthesis. The guard cells around each stoma regulate the pore aperture. Plants that carry out C4 photosynthesis are usually more resilient than C3 plants to stress, and their stomata operate over a lower dynamic range of CO2 within the leaf. What makes guard cells of C4 plants more responsive than those of C3 plants? We used gas exchange and electrophysiology, comparing stomatal kinetics of the C4 plant Gynandropsis gynandra and the phylogenetically related C3 plant Arabidopsis thaliana. We found, with varying CO2 and light, that Gynandropsis showed faster changes in stomata conductance and greater water use efficiency when compared with Arabidopsis. Electrophysiological analysis of the dominant K+ channels showed that the outward-rectifying channels, responsible for K+ loss during stomatal closing, were characterised by a greater maximum conductance and substantial negative shift in the voltage dependence of gating, indicating a reduced inhibition by extracellular K+ and enhanced capacity for K+ flux. These differences correlated with the accelerated stomata kinetics of Gynandropsis, suggesting that subtle changes in the biophysical properties of a key transporter may prove a target for future efforts to engineer C4 stomatal kinetics.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was funded by BBSRC grants BB/T006153/1, BB/T013508/1 and BB/W001217/1 to M.R.B and by a Lord Kelvin-Adam Smith PhD scholarship to F.A.L.S-A. |
| Status: | Early Online Publication |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Blatt, Professor Michael and Harvey, Professor Andy and Alvim, Mr Jonas and Lima Silva Alvim, Mrs Fernanda |
| Authors: | Silva-Alvim, F. A.L., Alvim, J. C., Harvey, A., and Blatt, M. R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Physics and Astronomy |
| Journal Name: | Plant, Cell and Environment |
| Publisher: | Wiley |
| ISSN: | 0140-7791 |
| ISSN (Online): | 1365-3040 |
| Published Online: | 27 November 2023 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Plant, Cell and Environment 2023 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
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