Early-responsive molecular signatures associated with halophytic adaptation in Sesuvium portulacastrum (L.)

Kulkarni, J. et al. (2024) Early-responsive molecular signatures associated with halophytic adaptation in Sesuvium portulacastrum (L.). Plant, Cell and Environment, 47(3), pp. 961-975. (doi: 10.1111/pce.14767) (PMID:38044749)

Abstract

Sesuvium portulacastrum (L.) is a halophyte, adapted to grow naturally under saline environments. The ability to use Na and K interchangeably indicated its facultative halophyte nature. No significant growth reduction occurs in seedlings up to 250 mM NaCl, except for curling of the youngest leaf. Within 8 h of salt treatment, seedlings accumulate proline, glycine betaine and other amino acids in both root and shoot. Despite a continued increase of tissue Na content, the number of differentially expressed genes (DEGs) decreases between 8 and 24 h of salt exposure, indicating transcriptional restoration after the initial osmotic challenge. At 8 h, upregulated genes mainly encode transporters and transcription factors, while genes in growth-related pathways such as photosynthesis and ribosome-associated biogenesis are suppressed. Overexpression of SpRAB18 (an ABA-responsive dehydrin), one of the most strongly induced DEGs, in soybean was found to increase biomass in control conditions and the growth benefit was maintained when plants were grown in 100 mM NaCl, indicating conservation of function in halophyte and glycophyte. An open-access transcriptome database “SesuviumKB” (https://cb.imsc.res.in/sesuviumkb/) was developed to involve the scientific community in wide-scale functional studies of S. portulacastrum genes, that could pave the way to engineer salt tolerance in crops.

• Item Type: Articles
• Additional Information: JK sincerely thank Department of Biotechnology (DBT) for providing financial assistance by awarding JRF and SRF. Parts of this work were funded by a DBT/BBSRC grant awarded to AKS (BT/IN/Indo-UK/PORI/02/AKS/2018-19) and to AA and PH (BBSRC grant BB/R019894/1,2).
• Keywords: Halophyte, transporters, transcription factors, salt tolerance, osmotic adjustment, abscisic acid.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Srivastava, Dr Ashish and Amtmann, Professor Anna and Herzyk, Dr Pawel
• Authors: Kulkarni, J., Sahoo, S. A., Herzyk, P., Barvkar, V. T., Kumar, S. A., Ravichandran, J., Samal, A., Amtmann, A., Borde, M., Suprasanna, P., and Srivastava, A. K.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Plant, Cell and Environment
• Publisher: Wiley
• ISSN: 0140-7791
• ISSN (Online): 1365-3040
• Published Online: 03 December 2023
• Copyright Holders: Copyright © 2023 John Wiley and Sons Ltd.
• First Published: First published in Plant, Cell and Environment 47(3):961-975
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
302195		Combining chemical priming and quantitative genetics to increase salt tolerance of soybean	Anna Amtmann	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/R019894/1	School of Molecular Biosciences