Pazmiño Betancourth, M. , Ochoa-Gutiérrez, V. , Ferguson, H. M. , González-Jiménez, M. , Wynne, K. , Baldini, F. and Childs, D. (2023) Evaluation of diffuse reflectance spectroscopy for predicting age, species, and cuticular resistance of Anopheles gambiae s.l under laboratory conditions. Scientific Reports, 13, 18499. (doi: 10.1038/s41598-023-45696-x) (PMID:37898634) (PMCID:PMC10613238)
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Abstract
Mid-infrared spectroscopy (MIRS) combined with machine learning analysis has shown potential for quick and efficient identification of mosquito species and age groups. However, current technology to collect spectra is destructive to the sample and does not allow targeting specific tissues of the mosquito, limiting the identification of other important biological traits such as insecticide resistance. Here, we assessed the use of a non-destructive approach of MIRS for vector surveillance, micro diffuse reflectance spectroscopy (µDRIFT) using mosquito legs to identify species, age and cuticular insecticide resistance within the Anopheles gambiae s.l. complex. These mosquitoes are the major vectors of malaria in Africa and the focus on surveillance in malaria control programs. Legs required significantly less scanning time and showed more spectral consistence compared to other mosquito tissues. Machine learning models were able to identify An. gambiae and An. coluzzii with an accuracy of 0.73, two ages groups (3 and 10 days old) with 0.77 accuracy and we obtained accuracy of 0.75 when identifying cuticular insecticide resistance. Our results highlight the potential of different mosquito tissues and µDRIFT as tools for biological trait identification on mosquitoes that transmit malaria. These results can guide new ways of identifying mosquito traits which can help the creation of innovative surveillance programs by adapting new technology into mosquito surveillance and control tools.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ochoa Gutierrez, Dr Victor and Childs, Dr David and Gonzalez Jimenez, Dr Mario and Pazmino Betancourth, Mr Mauro and Baldini, Dr Francesco and Wynne, Professor Klaas and Ferguson, Professor Heather |
| Authors: | Pazmiño Betancourth, M., Ochoa-Gutiérrez, V., Ferguson, H. M., González-Jiménez, M., Wynne, K., Baldini, F., and Childs, D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine College of Science and Engineering > School of Chemistry College of Science and Engineering > School of Engineering |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Research |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Scientific Reports 13:18499 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
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