Aslan, Y., McGleish, O., Reboud, J. and Cooper, J. M. (2023) Alignment-free construction of double emulsion droplet generation devices incorporating surface wettability contrast. Lab on a Chip, 23, 5173. (doi: 10.1039/D3LC00584D) (PMID:37966340)
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Abstract
Although polydimethylsiloxane (PDMS) is a versatile and easy-to-use material for microfluidics, its inherent hydrophobicity often necessitates specific hydrophilic treatment to fabricate microchip architectures for generating double emulsions. These additional processing steps often lead to increased complexity, potentially creating barriers to the wider use of promising microfluidic techniques. Here we describe an alignment-free spatial hydrophilic PDMS patterning technique to produce devices for the creation of double emulsions using combinations of PDMS and PDMS/surfactant bilayers. The technique enables us to achieve selective patterning and alignment-free bonding, producing reliable and reproducible W/O/W droplet emulsions. Our method involves processing device production in a vertical orientation, with the wetting transition contrast being achieved simply by imaging whilst adjusting the PDMS pouring speed (using a mobile phone, for example). We successfully obtain hydrophilic surfaces without distinguishable hydrophobic recovery using a range of surfactant concentrations. Droplet emulsions were produced with low coefficients of variation aligned with those generated with other, more complex, techniques (e.g. 3.8% and 3.1% for the inner and outer diameters, respectively). The process can be extended to liposome production whilst it could be applied to other fields, including reagent delivery, DNA amplification, and encapsulated cell studies for example.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by the Republic of Turkey Ministry of National Education (MoNE-1416/YLSY) and the United Kingdom Research and Innovation Engineering and Physical Sciences Research Council (EP/P001114/1 and EP/T021020/1). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cooper, Professor Jonathan and Reboud, Professor Julien and Aslan, Mr Yunus and McGleish, Miss Olivia |
| Creator Roles: | Aslan, Y.Conceptualization, Methodology, Formal analysis, Investigation, Data curation, Visualization, Writing – original draft, Writing – review and editing McGleish, O.Methodology, Formal analysis, Investigation, Data curation, Visualization, Writing – original draft, Writing – review and editing Reboud, J.Supervision, Writing – review and editing Cooper, J.Supervision, Writing – review and editing |
| Authors: | Aslan, Y., McGleish, O., Reboud, J., and Cooper, J. M. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Lab on a Chip |
| Publisher: | Royal Society of Chemistry |
| ISSN: | 1473-0197 |
| ISSN (Online): | 1473-0189 |
| Published Online: | 06 October 2023 |
| Copyright Holders: | Copyright © The Royal Society of Chemistry 2023 |
| First Published: | First published in Lab on a Chip 23:5173 |
| Publisher Policy: | Reproduced under a Creative Commons license |
| Data DOI: | 10.5525/gla.researchdata.1505 |
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