James, A. B. , Sharples, C., Laird, J., Armstrong, E. M., Guo, W., Tzioutziou, N., Zhang, R., Brown, J. W.S., Nimmo, H. and Jones, M. (2023) REVEILLE2 thermosensitive splicing: a molecular basis for the integration of nocturnal temperature information by the Arabidopsis circadian clock. New Phytologist, (doi: 10.1111/nph.19339) (PMID:37897048) (Early Online Publication)
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Abstract
• Cold stress is one of the major environmental factors that limit growth and yield of plants. However, it is still not fully understood how plants account for daily temperature fluctuations, nor how these temperature changes are integrated with other regulatory systems such as the circadian clock. • We demonstrate that REVEILLE2 undergoes alternative splicing after chilling that increases accumulation of a transcript isoform encoding a MYB-like transcription factor. We explore the biological function of REVEILLE2 in Arabidopsis thaliana using a combination of molecular genetics, transcriptomics, and physiology. • Disruption of REVEILLE2 alternative splicing alters regulatory gene expression, impairs circadian timing, and improves photosynthetic capacity. Changes in nuclear gene expression are particularly apparent in the initial hours following chilling, with chloroplast gene expression subsequently up-regulated. • The response of REVEILLE2 to chilling extends our understanding of plants immediate response to cooling. We propose that the circadian component REVEILLE2 restricts plants responses to nocturnal reductions in temperature, thereby enabling appropriate responses to daily environmental changes.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by UKRI/BBSRC (grants BB/P006868/1, BB/S005404/1, BB/P009751/1, BB/S020160/1) and the Scottish Government Rural and Environment Science and Analytical Services division (RESAS; grant number JHI-B1-2). |
| Status: | Early Online Publication |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Sharples, Mrs Chantal and Jones, Dr Matt and Armstrong, Dr Emily May and Laird, Ms Janet and Nimmo, Professor Hugh and James, Dr Allan |
| Authors: | James, A. B., Sharples, C., Laird, J., Armstrong, E. M., Guo, W., Tzioutziou, N., Zhang, R., Brown, J. W.S., Nimmo, H., and Jones, M. |
| Subjects: | Q Science > QK Botany |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | New Phytologist |
| Publisher: | Wiley |
| ISSN: | 0028-646X |
| ISSN (Online): | 1469-8137 |
| Published Online: | 28 October 2023 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in New Phytologist |
| Publisher Policy: | Reproduced under a Creative Commons license |
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