Nanoscatterer-assisted fluorescence amplification technique

Bonnefond, S., Reynaud, A., Cazareth, J., Abélanet, S., Vassalli, M. , Brau, F. and Lippi, G. L. (2023) Nanoscatterer-assisted fluorescence amplification technique. Nanomaterials, 13(21), 2875. (doi: 10.3390/nano13212875) (PMID:37947721)

Abstract

Weak fluorescence signals, which are important in research and applications, are often masked by the background. Different amplification techniques are actively investigated. Here, a broadband, geometry-independent and flexible feedback scheme based on the random scattering of dielectric nanoparticles allows the amplification of a fluorescence signal by partial trapping of the radiation within the sample volume. Amplification of up to a factor of 40 is experimentally demonstrated in ultrapure water with dispersed TiO2 nanoparticles (30 to 50 nm in diameter) and fluorescein dye at 200 μ mol concentration (pumped with 5 ns long, 3 mJ laser pulses at 490 nm). The measurements show a measurable reduction in linewidth at the emission peak, indicating that feedback-induced stimulated emission contributes to the large gain observed.

• Item Type: Articles
• Additional Information: Funding for this work has been obtained from the Région PACA (Appel à Projets Exploratoire 2013, ALLUMA project), from the Université de Nice-Sophia Antipolis (CSI ALLUMA project), from the Université Côte d’Azur (FEDERATE, NODES and BIOPHOTUCA projects) and from the CNRS (Mission pour l’Interdisciplinarité, AMFAL project). S. Bonnefond is recipient of a Doctoral Contract of the Université Côte d’Azur. M. Vassalli is grateful to the Fédération W. Döblin for a short-term Invited Professor position on the project New Paths in Fluorescence Microscopy.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Vassalli, Professor Massimo

Creator Roles

Vassalli, M.: Methodology, Formal analysis

• Authors: Bonnefond, S., Reynaud, A., Cazareth, J., Abélanet, S., Vassalli, M., Brau, F., and Lippi, G. L.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Nanomaterials
• Publisher: MDPI
• ISSN: 2079-4991
• ISSN (Online): 2079-4991
• Copyright Holders: Copyright: © 2023 by the authors
• First Published: First published in Nanomaterials 13(21): 2875
• Publisher Policy: Reproduced under a Creative Commons licence