Quantifying neutralising antibody responses against SARS‑CoV‑2 in dried blood spots (DBS) and paired sera

Roper, K. J. et al. (2023) Quantifying neutralising antibody responses against SARS‑CoV‑2 in dried blood spots (DBS) and paired sera. Scientific Reports, 13, 15014. (doi: 10.1038/s41598-023-41928-2) (PMID:37697014) (PMCID:PMC10495436)

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The ongoing SARS-CoV-2 pandemic was initially managed by non-pharmaceutical interventions such as diagnostic testing, isolation of positive cases, physical distancing and lockdowns. The advent of vaccines has provided crucial protection against SARS-CoV-2. Neutralising antibody (nAb) responses are a key correlate of protection, and therefore measuring nAb responses is essential for monitoring vaccine efficacy. Fingerstick dried blood spots (DBS) are ideal for use in large-scale sero-surveillance because they are inexpensive, offer the option of self-collection and can be transported and stored at ambient temperatures. Such advantages also make DBS appealing to use in resource-limited settings and in potential future pandemics. In this study, nAb responses in sera, venous blood and fingerstick blood stored on filter paper were measured. Samples were collected from SARS-CoV-2 acutely infected individuals, SARS-CoV-2 convalescent individuals and SARS-CoV-2 vaccinated individuals. Good agreement was observed between the nAb responses measured in eluted DBS and paired sera. Stability of nAb responses was also observed in sera stored on filter paper at room temperature for 28 days. Overall, this study provides support for the use of filter paper as a viable sample collection method to study nAb responses.

Item Type:Articles
Additional Information:This work was supported by the University of Liverpool. This research was funded in part by the National Institute for Health and Care Research (CO-CIN-01) and jointly by NIHR and UK Research and Innovation (CV220-169, MC_PC_19059).
Glasgow Author(s) Enlighten ID:Ho, Dr Antonia
Authors: Roper, K. J., Thomas, J., Albalawi, W., Maddocks, E., Dobson, S., Alshehri, A., Barone, F. G., Baltazar, M., Semple, M. G., Ho, A., Turtle, L., ISARIC4C Consortium, , Paxton, W. A., and Pollakis, G.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN (Online):2045-2322
Copyright Holders:Copyright © The Author(s) 2023
First Published:First published in Scientific Reports 13: 15014
Publisher Policy:Reproduced under a Creative Commons licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
310665ISARIC - Coronavirus Clinical Characterisation ConsortiumAntonia HoMedical Research Council (MRC)MC_PC_19059 - 9815274SII - Virology