Differential utilization of CD134 as a functional receptor by diverse strains of feline immunodeficiency virus

Willett, B.J., McMonagle, E.L., Ridha, S. and Hosie, M.J. (2006) Differential utilization of CD134 as a functional receptor by diverse strains of feline immunodeficiency virus. Journal of Virology, 80(7), pp. 3386-3394. (doi: 10.1128/JVI.80.7.3386-3394.2006)

Abstract

The feline homologue of CD134 (fCD134) is the primary binding receptor for feline immunodeficiency virus (FIV), targeting the virus preferentially to activated CD4+ helper T cells. However, with disease progression, the cell tropism of FIV broadens such that B cells and monocytes/macrophages become significant reservoirs of proviral DNA, suggesting that receptor utilization may alter with disease progression. We examined the receptor utilization of diverse strains of FIV and found that all strains tested utilized CD134 as the primary receptor. Using chimeric feline x human CD134 receptors, the primary determinant of receptor function was mapped to the first cysteine-rich domain (CRD1) of fCD134. For the PPR and B2542 strains, the replacement of CDR1 of fCD134 (amino acids 1 to 64) with human CD134 (hCD134) alone was sufficient to confer nearly optimal receptor function. However, evidence of differential utilization of CD134 was revealed, since strains GL8, CPGammer (CPG41), TM2, 0827, and NCSU1 required determinants in the region spanning amino acids 65 to 85, indicating that these strains may require a more stringent interaction for infection to proceed.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hosie, Professor Margaret and Willett, Professor Brian
• Authors: Willett, B.J., McMonagle, E.L., Ridha, S., and Hosie, M.J.
• Subjects: S Agriculture > SF Animal culture > SF600 Veterinary Medicine; Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Journal of Virology
• Journal Abbr.: J. Virol.
• ISSN: 0022-538X
• ISSN (Online): 1098-5514
• Copyright Holders: Copyright © 2006 American Society for Microbiology
• First Published: First published in Journal of Virology 80(7):3386-3394
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
36338	1	Rational Design of a Lentiviral Vaccine	Margaret Hosie	Medical Research Council (MRC)	G0300387	Centre for Virus Research