Surface modified piezoelectric co-polymer poly (vinylidene fluoride-trifluoroethylene) supports physiological extracellular matrices to enhance mesenchymal stem cell adhesion for nanoscale mechanical stimulation

Donnelly, H., Sprott, M. R. , Poudel, A., Campsie, P., Childs, P., Reid, S., Salmeron-Sanchez, M. and Biggs, M. (2023) Surface modified piezoelectric co-polymer poly (vinylidene fluoride-trifluoroethylene) supports physiological extracellular matrices to enhance mesenchymal stem cell adhesion for nanoscale mechanical stimulation. ACS Applied Materials and Interfaces, 15(44), pp. 50652-50662. (doi: 10.1021/acsami.3c05128) (PMID:37718477) (PMCID:PMC10636716)

Abstract

There is an unmet clinical need to provide viable bone grafts for clinical use. Autologous bone, one of the most commonly transplanted tissues, is often used but is associated with donor site morbidity. Tissue engineering strategies to differentiate an autologous cell source, such as mesenchymal stromal cells (MSCs), into a potential bone-graft material could help to fulfill clinical demand. However, osteogenesis of MSCs can typically require long culture periods that are impractical in a clinical setting and can lead to significant cost. Investigation into strategies that optimize cell production is essential. Here, we use the piezoelectric copolymer poly(vinylidene fluoride–trifluoroethylene) (PVDF-TrFE), functionalized with a poly(ethyl acrylate) (PEA) coating that drives fibronectin network formation, to enhance MSC adhesion and to present growth factors in the solid phase. Dynamic electrical cues are then incorporated, via a nanovibrational bioreactor, and the MSC response to electromechanical stimulation is investigated.

• Item Type: Articles
• Additional Information: This work was supported by UKRI BBSRC Grant BB/ P00220X/1.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Sprott, Dr Mark and Childs, Dr Peter and Donnelly, Dr Hannah and Reid, Professor Stuart
• Authors: Donnelly, H., Sprott, M. R., Poudel, A., Campsie, P., Childs, P., Reid, S., Salmeron-Sanchez, M., and Biggs, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Applied Materials and Interfaces
• Publisher: American Chemical Society
• ISSN: 1944-8244
• ISSN (Online): 1944-8252
• Published Online: 17 September 2023
• Copyright Holders: Copyright © 2023 The Authors
• First Published: First published in ACS Applied Materials and Interfaces 15(44):50652–50662
• Publisher Policy: Reproduced under a Creative Commons license

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
173494		Rapid Bone Graft Synthesis Through Dual Piezoelectric/Nanomechaniocal Stimulation	Matthew Dalby	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/P00220X/1	SMB - Molecular Biosciences