Mitokyne: a ratiometric Raman probe for mitochondrial pH

Wilson, L. T. , Tipping, W. J., Wetherill, C., Henley, Z., Faulds, K., Graham, D., Mackay, S. P. and Tomkinson, N. C. O. (2021) Mitokyne: a ratiometric Raman probe for mitochondrial pH. Analytical Chemistry, 93(37), pp. 12786-12792. (doi: 10.1021/acs.analchem.1c03075) (PMID:34505518)

Abstract

Mitochondrial pH (pHmito) is intimately related to mitochondrial function, and aberrant values for pHmito are linked to several disease states. We report the design, synthesis, and application of mitokyne 1—the first small molecule pHmito sensor for stimulated Raman scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pHmito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.

• Item Type: Articles
• Additional Information: We thank the University of Strathclyde, the EPSRC (EP/TR512114/1), and GlaxoSmithKline for financial support.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Wilson, Mr Liam
• Authors: Wilson, L. T., Tipping, W. J., Wetherill, C., Henley, Z., Faulds, K., Graham, D., Mackay, S. P., and Tomkinson, N. C. O.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Analytical Chemistry
• Publisher: American Chemical Society
• ISSN: 0003-2700
• ISSN (Online): 1520-6882
• Published Online: 10 September 2021
• Copyright Holders: Copyright © 2021 American Chemical Society
• First Published: First published in Analytical Chemistry 93(37):12786-12792
• Publisher Policy: Reproduced under a Creative Commons license