Barnett, S. C. , Johnstone, S. A. and Lindsay, S. L. (2013) Comparative Study of Human Mesenchymal Stem Cells Isolated from the Olfactory System and Bone Marrow: Effects on Myelination. Glia 61(S1):s192. Meeting Abstract: XI European Meeting on Glial Cells in Health and Disease, Berlin, Germany, 3-6th July 2013. (doi: 10.1002/glia.22530)
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Abstract
Mesenchymal stem cells (MSCs) have been proposed as candidates for transplant mediated repair in multiple sclerosis (MS). Clinical trials are ongoing using autologous systemic transplantation of bone marrow derived MSCs into relapsing remitting, secondary progressive and primary progressive MS. Previously we have shown that rat and adult human olfactory mucosa (OM) contain a mesenchymal-like stem that resides in the lamina propria (LP-MSCs). Since the olfactory system is one of the only CNS tissues that promotes neurogenesis throughout life, cells from olfactory biopsies have been proposed as excellent candidates for CNS repair. Thus, we have made a detailed comparison of MSCs isolated from bone marrow with MSCs isolated from the OM. Using immunocytochemistry and q-PCR, purified human LP-MSCs expressed typical bone marrow derived (BM-) MSC markers, including CD90, CD105, CD73, CD166, CD271, NG2, fibronectin and STRO-1, however they expressed more nestin mRNA. LP-MSCs formed spheres, were clonogenic and differentiated into bone and fat. Comparative micro(mi)RNA-based fingerprinting (SistemQCtm from Sistemics) demonstrated 64% homology between the two MSCs, with only 26 differentially expressed miRNAs. LP-MSC conditioned medium (CM) promoted oligodendrocyte precursor cell proliferation and induced a highly branched morphology. Both LP and BM-MSCs promoted OPC proliferation and differentiation, but only myelinating cultures treated with CM from LP and not BM-MSCs had a significant increase in myelination. Comparison with fibroblasts and contaminating OM fibroblast-like cells showed the pro-myelination effect was LP-MSC specific. Preliminary in vivo cell transplantation studies suggest LP-MSCs can promote many neurites into the lesion and myelination around the lesion may be increased. Thus, LP-MSCs harvested from human OM biopsies may be an important candidate for cell transplantation by promoting CMS myelination.
| Item Type: | Conference or Workshop Item |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Lindsay, Dr Susan and Barnett, Professor Susan |
| Authors: | Barnett, S. C., Johnstone, S. A., and Lindsay, S. L. |
| Subjects: | Q Science > Q Science (General) |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Published Online: | 13 June 2013 |
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