Multiplexed biosensing of proteins and virions with disposable plasmonic assays

Wallace, S. et al. (2023) Multiplexed biosensing of proteins and virions with disposable plasmonic assays. ACS Sensors, 8(9), pp. 3338-3348. (doi: 10.1021/acssensors.2c02238) (PMID:37610841) (PMCID:PMC10521139)

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Our growing ability to tailor healthcare to the needs of individuals has the potential to transform clinical treatment. However, the measurement of multiple biomarkers to inform clinical decisions requires rapid, effective, and affordable diagnostics. Chronic diseases and rapidly evolving pathogens in a larger population have also escalated the need for improved diagnostic capabilities. Current chemical diagnostics are often performed in centralized facilities and are still dependent on multiple steps, molecular labeling, and detailed analysis, causing the result turnaround time to be over hours and days. Rapid diagnostic kits based on lateral flow devices can return results quickly but are only capable of detecting a handful of pathogens or markers. Herein, we present the use of disposable plasmonics with chiroptical nanostructures as a platform for low-cost, label-free optical biosensing with multiplexing and without the need for flow systems often required in current optical biosensors. We showcase the detection of SARS-CoV-2 in complex media as well as an assay for the Norovirus and Zika virus as an early developmental milestone toward high-throughput, single-step diagnostic kits for differential diagnosis of multiple respiratory viruses and any other emerging diagnostic needs. Diagnostics based on this platform, which we term “disposable plasmonics assays,” would be suitable for low-cost screening of multiple pathogens or biomarkers in a near-point-of-care setting.

Item Type:Articles
Additional Information:The authors would like to acknowledge the support from EPSRC through grants EP/S001514/1 and EP/S029168/1 and the QuantIC funding scheme and MRC through a fellowship (MR/N008618/1 and MR/V035789/1), MRC grants (MC_PC_19026 and MC_PC_21023), and the Wellcome Trust Early Concepts in Development Scheme (219390/Z/19/Z). The authors acknowledge that parts of the experimental work were carried out by CRUSH: COVID-19 Antiviral Drug Screening and Resistance Hub at the MRC-University of Glasgow Centre for Virus Research (CVR). Funding for this research was supported by a LifeArc COVID-19 award. The ZIKV-related work was funded by the Department of Health and Social Care using U.K. Aid funding and is managed by the NIHR (G.D.L. and A.H.P.) and by the U.K. Medical Research Council grant MC_UU12014/2 (A.H.P.).
Glasgow Author(s) Enlighten ID:De Lorenzo, Dr Giuditta and Vijayakrishnan, Dr Swetha and Samardzhieva, Ms Iliyana and Gadegaard, Professor Nikolaj and Kakkar, Dr Tarun and Cole, Ms Sarah and Karimullah, Dr Affar and Hutchinson, Dr Edward and Wallace, Ms Stephanie and Lapthorn, Dr Adrian and Kartau, Mr Martin and Patel, Professor Arvind and Davis, Dr Chris and Kadodwala, Professor Malcolm and Szemiel, Dr Agnieszka
Authors: Wallace, S., Kartau, M., Kakkar, T., Davis, C., Szemiel, A., Samardzhieva, I., Vijayakrishnan, S., Cole, S., De Lorenzo, G., Maillart, E., Gautier, K., Lapthorn, A. J., Patel, A., Gadegaard, N., Kadodwala, M., Hutchinson, E., and Karimullah, A. S.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
College of Science and Engineering > School of Chemistry
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Sensors
Publisher:American Chemical Society
ISSN (Online):2379-3694
Published Online:23 August 2023
Copyright Holders:Copyright © 2023 The Authors
First Published:First published in ACS Sensors 8(9):3338-3348
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
308165Institutional Translation Partnership AwardGerard GrahamWellcome Trust (WELLCOTR)219390/Z/19/ZMVLS - College Senior Management
312928MRC Transition Support Award CDAEdward HutchinsonMedical Research Council (MRC)MR/V035789/1SII - Virology
172398Functional investigations of the influenza virus proteomeEdward HutchinsonMedical Research Council (MRC)MR/N008618/1SII - MRC-UofG Centre for Virus Research
304762Meta-Smart: Dynamic hybrid bio-plasmonic metamaterials.Malcolm KadodwalaEngineering and Physical Sciences Research Council (EPSRC)EP/S029168/1Chemistry
302406High-Throughput Diagnostics with Chiral Plasmonic AssaysAffar KarimullahEngineering and Physical Sciences Research Council (EPSRC)EP/S001514/1Chemistry