Genetic diversity of Brazilian isolates of feline immunodeficiency virus

Teixeira, B. M., Logan, N., Cruz, J.C.M., Reis, J.K.P., Brandão, P.E., Richtzenhain, L.J., Hagiwara, M.K., Willett, B.J. and Hosie, M.J. (2010) Genetic diversity of Brazilian isolates of feline immunodeficiency virus. Archives of Virology, 155(3), pp. 379-384. (doi: 10.1007/s00705-009-0587-2)

Abstract

We isolated Feline immunodeficiency virus (FIV) from three adult domestic cats, originating from two open shelters in Brazil. Viruses were isolated from PBMC following co-cultivation with the feline T-lymphoblastoid cell line MYA-1. All amplified env gene products were cloned directly into pGL8MYA. The nucleic acid sequences of seven clones were determined and then compared with those of previously described isolates. The sequences of all of the Brazilian virus clones were distinct and phylogenetic analysis revealed that all belong to subtype B. Three variants isolated from one cat and two variants were isolated from each of the two other cats, indicating that intrahost diversity has the potential to pose problems for the treatment and diagnosis of FIV infection.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hosie, Professor Margaret and Willett, Professor Brian
• Authors: Teixeira, B. M., Logan, N., Cruz, J.C.M., Reis, J.K.P., Brandão, P.E., Richtzenhain, L.J., Hagiwara, M.K., Willett, B.J., and Hosie, M.J.
• Subjects: Q Science > QR Microbiology > QR355 Virology
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Archives of Virology
• Publisher: Springer Wein
• ISSN: 0304-8608
• ISSN (Online): 1432-8798
• Published Online: 20 January 2010

Related URLs

• PubMed

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
36338	1	Rational Design of a Lentiviral Vaccine	Margaret Hosie	Medical Research Council (MRC)	G0300387	Centre for Virus Research