OnGuard3e: a predictive, ecophysiology‐ready tool for gas exchange and photosynthesis research

Nguyen, T.-H. , Silva-Alvim, F. A. L., Hills, A. and Blatt, M. R. (2023) OnGuard3e: a predictive, ecophysiology‐ready tool for gas exchange and photosynthesis research. Plant, Cell and Environment, 46(11), pp. 3644-3658. (doi: 10.1111/pce.14674) (PMID:37498151)

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Abstract

Gas exchange across the stomatal pores of leaves is a focal point in studies of plant-environmental relations. Stomata regulate atmospheric exchange with the inner air spaces of the leaf. They open to allow CO2 entry for photosynthesis and close to minimize water loss. Models that focus on the phenomenology of stomatal conductance generally omit the mechanics of the guard cells that regulate the pore aperture. The OnGuard platform fills this gap and offers a truly mechanistic approach with which to analyse stomatal gas exchange, whole-plant carbon assimilation and water-use efficiency. Previously, OnGuard required specialist knowledge of membrane transport, signalling and metabolism. Here we introduce OnGuard3e, a software package accessible to ecophysiologists and membrane biologists alike. We provide a brief guide to its use and illustrate how the package can be applied to explore and analyse stomatal conductance, assimilation and water use efficiencies, addressing a range of experimental questions with truly predictive outputs.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hills, Mr Adrian and Nguyen, Thanh and Blatt, Professor Michael and Lima Silva Alvim, Mrs Fernanda
Authors: Nguyen, T.-H., Silva-Alvim, F. A. L., Hills, A., and Blatt, M. R.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Plant, Cell and Environment
Publisher:Wiley
ISSN:0140-7791
ISSN (Online):1365-3040
Published Online:27 July 2023
Copyright Holders:Copyright © 2023 The Authors
First Published:First published in Plant, Cell and Environment 46(11):3644-3658
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190663Stomatal-based systems analysis of water use efficiencyMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/L001276/1MCSB - Plant Sciences
170646Developing a synthetic approach to manipulating guard cell membrane transport and stomatal controlMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/L019205/1MCSB - Plant Sciences
170599Analysing GORK clustering for enhanced stomatal controlMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/M001601/1MCSB - Plant Sciences
172696Synthesis of Microcompartments in Plants for Enhanced Carbon FixationMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/N01832X/1MCSB - Plant Sciences
307708BlinkGUARDMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/T006153/1School of Molecular Biosciences