Karnik, R. and Blatt, M. R. (2023) Analyzing protein–protein interactions using the split-ubiquitin system. In: Mukhtar, S. (ed.) Protein-Protein Interactions: Methods and Protocols. Series: Methods in molecular biology (2690). Humana Press: New York, NY, pp. 23-36. ISBN 9781071633267 (doi: 10.1007/978-1-0716-3327-4_3)
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Abstract
The split-ubiquitin technology was developed over 20 years ago as an alternative to Gal4-based, yeast-two-hybrid methods to identify interacting protein partners. With the introduction of mating-based methods for split-ubiquitin screens, the approach has gained broad popularity because of its exceptionally high transformation efficiency, utility in working with full-length membrane proteins, and positive selection with little interference from spurious interactions. Recent advances now extend these split-ubiquitin methods to the analysis of interactions between otherwise soluble proteins and tripartite protein interactions.
| Item Type: | Book Sections |
|---|---|
| Additional Information: | Funding from the UK Biotechnology and Biological Sciences Research Council and the Royal Society of London is acknowledged. |
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Karnik, Dr Rucha and Blatt, Professor Michael |
| Authors: | Karnik, R., and Blatt, M. R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Publisher: | Humana Press |
| ISBN: | 9781071633267 |
| Published Online: | 15 July 2023 |
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