Leggewie, M. et al. (2023) The Aedes aegypti RNA interference response against Zika virus in the context of co-infection with dengue and chikungunya viruses. PLoS Neglected Tropical Diseases, 17(7), e0011456. (doi: 10.1371/journal.pntd.0011456) (PMID:37440582) (PMCID:PMC10343070)
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Abstract
Since its detection in 2015 in Brazil, Zika virus (ZIKV) has remained in the spotlight of international public health and research as an emerging arboviral pathogen. In addition to single infection, ZIKV may occur in co-infection with dengue (DENV) and chikungunya (CHIKV) viruses, with whom ZIKV shares geographic distribution and the mosquito Aedes aegypti as a vector. The main mosquito immune response against arboviruses is RNA interference (RNAi). It is unknown whether or not the dynamics of the RNAi response differ between single arboviral infections and co-infections. In this study, we investigated the interaction of ZIKV and DENV, as well as ZIKV and CHIKV co-infections with the RNAi response in Ae. aegypti. Using small RNA sequencing, we found that the efficiency of small RNA production against ZIKV -a hallmark of antiviral RNAi—was mostly similar when comparing single and co-infections with either DENV or CHIKV. Silencing of key antiviral RNAi proteins, showed no change in effect on ZIKV replication when the cell is co-infected with ZIKV and DENV or CHIKV. Interestingly, we observed a negative effect on ZIKV replication during CHIKV co-infection in the context of Ago2-knockout cells, though his effect was absent during DENV co-infection. Overall, this study provides evidence that ZIKV single or co-infections with CHIKV or DENV are equally controlled by RNAi responses. Thus, Ae. aegypti mosquitoes and derived cells support co-infections of ZIKV with either CHIKV or DENV to a similar level than single infections, as long as the RNAi response is functional.
| Item Type: | Articles |
|---|---|
| Additional Information: | Funding: This research was supported by the German Centre for Infection Research (DZIF) (TTU 01.701 and TTU 01.708) (ES), the European Union’s Horizon 2020 Research and Innovation Program under ZIKAlliance Grant Agreement No 734548 (ES, ABF, AK), the UK MRC (MC_UU_12014/8) (AK). This publication was supported by the European Virus Archive goes Global (EVAg) project that has received funding from the European Union´s Horizon 2020 research and innovation programme under grant agreement No 653316. This work was supported by the DFG Research Infrastructure NGS_CC (project 407495230 to JF) as part of the Next Generation Sequencing Competence Network (project 423957469 to JF). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Vattipally, Dr Sreenu and Kohl, Professor Alain |
| Creator Roles: | Sreenu, V. B.Data curation, Formal analysis, Software, Visualization, Writing – review and editing Kohl, A.Conceptualization, Funding acquisition, Methodology, Supervision, Writing – review and editing |
| Authors: | Leggewie, M., Scherer, C., Altinli, M., Gestuveo, R. J., Sreenu, V. B., Fuss, J., Vazeille, M., Mousson, L., Badusche, M., Kohl, A., Failloux, A.-B., and Schnettler, E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | PLoS Neglected Tropical Diseases |
| Publisher: | Public Library of Science |
| ISSN: | 1935-2727 |
| ISSN (Online): | 1935-2735 |
| Published Online: | 13 July 2023 |
| Copyright Holders: | Copyright © 2023 Leggewie et al. |
| First Published: | First published in PLoS Neglected Tropical Diseases 17(7):e0011456 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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