Photocrosslinked gelatin methacryloyl (GelMA)/hyaluronic acid methacryloyl (HAMA) composite scaffold using anthocyanidin as a photoinitiator for bone tissue regeneration

Lin, Y.-C., Nixon, E. J., Wang, H.-Y., Dhawan, U. , Huang, Y.-W., Huang, S.-H., Jiang, C.-P., Kuo, Y.-J. and Chung, R.-J. (2023) Photocrosslinked gelatin methacryloyl (GelMA)/hyaluronic acid methacryloyl (HAMA) composite scaffold using anthocyanidin as a photoinitiator for bone tissue regeneration. ACS Applied Polymer Materials, 5(8), pp. 6012-6021. (doi: 10.1021/acsapm.3c00710)

Abstract

Gelatin and hyaluronic acid are natural biopolymers that are important components of soft tissues in the human body, and both have been used in several biomedical applications. To achieve controllable mechanical and degradation properties of gelatin and hyaluronic acid, methacrylic acid has been used to modify photocrosslinkable gelatin methacryloyl (GelMA) and hyaluronic acid methacryloyl (HAMA). Irgacure 2959 (I2959) and anthocyanidin have been used as photoinitiators for comparison. Finally, the photocrosslinked GelMA/HAMA composite scaffold has been prepared for bone tissue regeneration. The composite scaffolds were prepared using mold casting and photocrosslinking techniques. Specific ratios of GelMA and HAMA reagents were mixed properly with I2959 or anthocyanidin photoinitiators. Samples were examined with chemical and material, in vitro, and in vivo evaluations. Furthermore, two photoinitiators, I2959 and anthocyanidin, can crosslink the GelMA/HAMA composite scaffold. A 1% HAMA incorporation can significantly decrease the degradation of the GelMA/HAMA scaffold compared with GelMA only. Also, HAMA participation can improve cell viability and promote bone-related cell differentiation and tissue regeneration (bone formation) without any apparent adverse reactions in vivo.

• Item Type: Articles
• Additional Information: This research was supported financially by the Ministry of Science and Technology of Taiwan (MOST 109-2622-E-027-003-CC3 and MOST 110-2622-E-027-015) and the National Taipei University of Technology-Mackay Memorial Hospital Joint Research Program (NTUT-MMH-111-01).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Dhawan, Dr Udesh
• Authors: Lin, Y.-C., Nixon, E. J., Wang, H.-Y., Dhawan, U., Huang, Y.-W., Huang, S.-H., Jiang, C.-P., Kuo, Y.-J., and Chung, R.-J.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Applied Polymer Materials
• Publisher: American Chemical Society
• ISSN: 2637-6105
• ISSN (Online): 2637-6105
• Published Online: 18 July 2023
• Copyright Holders: Copyright © 2023 American Chemical Society
• First Published: First published in ACS Applied Polymer Materials 5(8): 6012-6021
• Publisher Policy: Reproduced in accordance with the publisher copyright policy

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