Inhibition of RNA degradation integrates the metabolic signals induced by osmotic stress into the Arabidopsis circadian system

Prasetyaningrum, P., Litthauer, S., Vegliani, F., Battle, M. W., Wood, M. W., Liu, X., Dickson, C. and Jones, M. A. (2023) Inhibition of RNA degradation integrates the metabolic signals induced by osmotic stress into the Arabidopsis circadian system. Journal of Experimental Botany, 74(18), pp. 5805-5819. (doi: 10.1093/jxb/erad274) (PMID:37453132) (PMCID:PMC10540740)

Abstract

The circadian clock system acts as an endogenous timing reference that coordinates many metabolic and physiological processes in plants. Previous studies have shown that the application of osmotic stress delays circadian rhythms via 3’-Phospho-Adenosine 5’-Phosphate (PAP), a retrograde signalling metabolite that is produced in response to redox stress within organelles. PAP accumulation leads to the inhibition of EXORIBONUCLEASEs (XRNs), which are responsible for RNA degradation. Interestingly, we are now able to demonstrate that post-transcriptional processing is crucial for the circadian response to osmotic stress. Our data show that osmotic stress increases the stability of specific circadian RNAs, suggesting that RNA metabolism plays a vital role in circadian clock coordination during drought. Inactivation of XRN4 is sufficient to extend circadian rhythms as part of this response, with PRR7 and LWD1 identified as transcripts that are post-transcriptionally regulated to delay circadian progression.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Liu, Xinmeng and Vegliani, Franco and Prasetyaningrum, Dr Putri and Jones, Dr Matt and Dickson, Mrs Cathryn and Battle, Dr Martin
• Authors: Prasetyaningrum, P., Litthauer, S., Vegliani, F., Battle, M. W., Wood, M. W., Liu, X., Dickson, C., and Jones, M. A.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Journal of Experimental Botany
• Publisher: Oxford University Press
• ISSN: 0022-0957
• ISSN (Online): 1460-2431
• Published Online: 15 July 2023
• Copyright Holders: Copyright © 2023 The Authors
• First Published: First published in Journal of Experimental Botany 74(18): 5805–5819
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
307958		How does PAP, a stress-induced metabolite, regulate gene expression?	Matthew Jones	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/S005404/1	School of Molecular Biosciences