UHPLC-HRMS spectrometric analysis: method validation and plasma and urinary metabolite identification after mango pulp intake

Cáceres-Jiménez, S., Rodríguez-Solana, R., Dobani, S., Pourshahidi, K., Gill, C., Moreno-Rojas, J. M., Almutairi, T. M., Crozier, A. and Pereira-Caro, G. (2023) UHPLC-HRMS spectrometric analysis: method validation and plasma and urinary metabolite identification after mango pulp intake. Journal of Agricultural and Food Chemistry, 71(30), pp. 11520-11533. (doi: 10.1021/acs.jafc.3c03846) (PMID:37471325)

Abstract

After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Crozier, Professor Alan
• Authors: Cáceres-Jiménez, S., Rodríguez-Solana, R., Dobani, S., Pourshahidi, K., Gill, C., Moreno-Rojas, J. M., Almutairi, T. M., Crozier, A., and Pereira-Caro, G.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
• Journal Name: Journal of Agricultural and Food Chemistry
• Publisher: American Chemical Society
• ISSN: 0021-8561
• ISSN (Online): 1520-5118
• Published Online: 20 July 2023
• Copyright Holders: Copyright © 2023 American Chemical Society
• First Published: First published in Journal of Agricultural and Food Chemistry 71(30):11520-11533
• Publisher Policy: Reproduced in accordance with the publisher copyright policy