McInnes, K. J., van der Hooft, J. J.J. , Sharma, A., Herzyk, P. , Hundleby, P. A.C., Schoonbeek, H.-J., Amtmann, A. , Ridout, C. and Jenkins, G. I. (2023) Overexpression of Brassica napus COMT1 in Arabidopsis heightens UV-B-mediated resistance to Plutella xylostella herbivory. Photochemical and Photobiological Sciences, 22, pp. 2341-2356. (doi: 10.1007/s43630-023-00455-9) (PMID:37505444) (PMCID:PMC10509076)
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Abstract
UV-B radiation regulates numerous morphogenic, biochemical and physiological responses in plants, and can stimulate some responses typically associated with other abiotic and biotic stimuli, including invertebrate herbivory. Removal of UV-B from the growing environment of various plant species has been found to increase their susceptibility to consumption by invertebrate pests, however, to date, little research has been conducted to investigate the effects of UV-B on crop susceptibility to field pests. Here, we report findings from a multi-omic and genetic-based study investigating the mechanisms of UV-B-stimulated resistance of the crop, Brassica napus (oilseed rape), to herbivory from an economically important lepidopteran specialist of the Brassicaceae, Plutella xylostella (diamondback moth). The UV-B photoreceptor, UV RESISTANCE LOCUS 8 (UVR8), was not found to mediate resistance to this pest. RNA-Seq and untargeted metabolomics identified components of the sinapate/lignin biosynthetic pathway that were similarly regulated by UV-B and herbivory. Arabidopsis mutants in genes encoding two enzymes in the sinapate/lignin biosynthetic pathway, CAFFEATE O-METHYLTRANSFERASE 1 (COMT1) and ELICITOR-ACTIVATED GENE 3–2 (ELI3-2), retained UV-B-mediated resistance to P. xylostella herbivory. However, the overexpression of B. napus COMT1 in Arabidopsis further reduced plant susceptibility to P. xylostella herbivory in a UV-B-dependent manner. These findings demonstrate that overexpression of a component of the sinapate/lignin biosynthetic pathway in a member of the Brassicaceae can enhance UV-B-stimulated resistance to herbivory from P. xylostella.
| Item Type: | Articles |
|---|---|
| Additional Information: | Project funded by the BBSRC Crop Improvement Research Club (CIRC) grant number BB/I017518/1. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Jenkins, Professor Gareth and Van Der Hooft, Mr Justin and Amtmann, Professor Anna and Herzyk, Dr Pawel |
| Authors: | McInnes, K. J., van der Hooft, J. J.J., Sharma, A., Herzyk, P., Hundleby, P. A.C., Schoonbeek, H.-J., Amtmann, A., Ridout, C., and Jenkins, G. I. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Photochemical and Photobiological Sciences |
| Publisher: | Springer |
| ISSN: | 1474-905X |
| ISSN (Online): | 1474-9092 |
| Published Online: | 28 July 2023 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Photochemical and Photobiological Sciences 22:2341–2356 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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