MicroLED biosensor with colloidal quantum dots and smartphone detection

Bruce, N., Farrell, F., Xie, E., Scullion, M. G. , Haughey, A.-M., Gu, E., Dawson, M. D. and Laurand, N. (2023) MicroLED biosensor with colloidal quantum dots and smartphone detection. Biomedical Optics Express, 14(3), pp. 1107-1118. (doi: 10.1364/BOE.478276) (PMID:36950244) (PMCID:PMC10026578)

Abstract

A fluorescence sensor with the capability for spatially multiplexed measurements utilizing smartphone detection is presented. Bioconjugated quantum dots are used as the fluorescent tag and are excited using a blue-emitting microLED (µLED). The 1-dimensional GaN µLED array is butt-coupled to one edge of the glass slide to take advantage of total internal reflection fluorescence (TIRF) principles. The bioassays on the top surface of the glass waveguide are excited and the resultant fluorescence is detected with the smartphone. The red, green, and blue channels of the digital image are utilized to spectrally separate the excitation light from the fluorescence for analysis. Using a biotin-functionalized glass slide as proof of principle, we have shown that streptavidin conjugated quantum dots can be detected down to a concentration of 8 nM.

• Item Type: Articles
• Additional Information: Funding: EPSRC Centre for Doctoral Training in Applied Photonics (EP/L01596X/1); Leverhulme Trust (RL-19-038).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Scullion, Dr Mark
• Authors: Bruce, N., Farrell, F., Xie, E., Scullion, M. G., Haughey, A.-M., Gu, E., Dawson, M. D., and Laurand, N.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Journal Name: Biomedical Optics Express
• Publisher: Optica Publishing Group
• ISSN: 2156-7085
• ISSN (Online): 2156-7085
• Published Online: 10 February 2023
• Copyright Holders: Copyright © 2023 The Authors
• First Published: First published in Biomedical Optics Express 14(3): 1107-1118
• Publisher Policy: Reproduced under a Creative Commons License
• Data DOI: 10.15129/951ce0ce-232e-4033-8c18-ce6ab5d774d9

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
173149		The EPSRC Centre for Doctoral Training in applied photonics	Andrew Harvey	Engineering and Physical Sciences Research Council (EPSRC)	EP/L01596X/1	P&S - Physics & Astronomy