Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering

Oliver-Cervelló, L., Martin-Gómez, H., Gonzalez-Garcia, C., Salmeron-Sanchez, M. , Ginebra, M.-P. and Mas-Moruno, C. (2023) Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering. Frontiers in Bioengineering and Biotechnology, 11, 1192436. (doi: 10.3389/fbioe.2023.1192436) (PMID:37324414) (PMCID:PMC10267393)

Abstract

Mimicking bone extracellular matrix (ECM) is paramount to develop novel biomaterials for bone tissue engineering. In this regard, the combination of integrin-binding ligands together with osteogenic peptides represents a powerful approach to recapitulate the healing microenvironment of bone. In the present work, we designed polyethylene glycol (PEG)-based hydrogels functionalized with cell instructive multifunctional biomimetic peptides (either with cyclic RGD-DWIVA or cyclic RGD-cyclic DWIVA) and cross-linked with matrix metalloproteinases (MMPs)-degradable sequences to enable dynamic enzymatic biodegradation and cell spreading and differentiation. The analysis of the intrinsic properties of the hydrogel revealed relevant mechanical properties, porosity, swelling and degradability to engineer hydrogels for bone tissue engineering. Moreover, the engineered hydrogels were able to promote human mesenchymal stem cells (MSCs) spreading and significantly improve their osteogenic differentiation. Thus, these novel hydrogels could be a promising candidate for applications in bone tissue engineering, such as acellular systems to be implanted and regenerate bone or in stem cells therapy.

• Item Type: Articles
• Additional Information: This work was supported by the Spanish State Research Agency (PID 2020-114019RB-I00/AEI/10.13039/501100011033), the AGAUR (2021 SGR 01368 and FI-2018 predoctoral fellowship of LO-C) and the Generalitat of Catalunya (ICREA Academia Award of MP-G). This work has also received funding from the European Union’s Horizon 2020 research and innovation Programme under the Marie Sklodowska-Curie Grant Agreement No. 872869 (project Bio-Tune, RISE).
• Keywords: Osteogenic differentiation, biomimetic peptides, functionalization, hydrogel, multifunctionality, DWIVA.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Gonzalez Garcia, Dr Cristina
• Authors: Oliver-Cervelló, L., Martin-Gómez, H., Gonzalez-Garcia, C., Salmeron-Sanchez, M., Ginebra, M.-P., and Mas-Moruno, C.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Research Centre: Mazumdar-Shaw Advanced Research Centre (ARC) > Technologies Touching Life
• Journal Name: Frontiers in Bioengineering and Biotechnology
• Publisher: Frontiers Media
• ISSN: 2296-4185
• ISSN (Online): 2296-4185
• Copyright Holders: Copyright © 2023 Oliver-Cervelló, Martin-Gómez, Gonzalez-Garcia, Salmeron-Sanchez, Ginebra and Mas-Moruno
• First Published: First published in Frontiers in Bioengineering and Biotechnology 11: 1192436
• Publisher Policy: Reproduced under a Creative Commons License