Ibarra-Chavez, R., Reboud, J. , Penades, J. R., and Cooper, J. M. (2023) Phage-inducible chromosomal islands as a diagnostic platform to capture and detect bacterial pathogens. Advanced Science, 10(24), 2301643. (doi: 10.1002/advs.202301643) (PMID:37358000) (PMCID:PMC10460865)
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Abstract
Phage-inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user-friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL−1. In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell-free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work and R.I.-C. were supported by a research grant from the CONACYT (Scholarship 329695/384590). This study was supported by the Medical Research Council (UK) grants MC_PC_17160 to R.I.-C and J.R.P, MR/S00940X/1 and MR/M003876/1 to J.R.P., the UK Global Challenges Research Fund, the Scottish Funding Council, and Engineering and Physical Sciences Research Council (EPSRC) Institutional Support Fund EP/R512813/1 to J.M.C., as well by EP/R01437X/1 also supported by the National Institute for Health Research to J.R. and J.M.C. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ibarra Chavez, Mr Rodrigo and Cooper, Professor Jonathan and Penades, Prof Jose R and Reboud, Professor Julien |
| Authors: | Ibarra-Chavez, R., Reboud, J., Penades, J. R.,, and Cooper, J. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Advanced Science |
| Publisher: | Wiley |
| ISSN: | 2198-3844 |
| ISSN (Online): | 2198-3844 |
| Published Online: | 26 June 2023 |
| Copyright Holders: | Copyright © 2023 The Authors. |
| First Published: | First published in Advanced Science 10(24):2301643 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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