Durrani, Z., Kinnaird, J., Cheng, C. W., Brühlmann, F., Capewell, P. , Jackson, A., Larcombe, S., Olias, P., Weir, W. and Shiels, B. (2023) A parasite DNA binding protein with potential to influence disease susceptibility acts as an analogue of mammalian HMGA transcription factors. PLoS ONE, 18(6), e0286526. (doi: 10.1371/journal.pone.0286526) (PMID:37276213) (PMCID:PMC10241358)
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Abstract
Intracellular pathogens construct their environmental niche, and influence disease susceptibility, by deploying factors that manipulate infected host cell gene expression. Theileria annulata is an important tick-borne parasite of cattle that causes tropical theileriosis. Excellent candidates for modulating host cell gene expression are DNA binding proteins bearing AT-hook motifs encoded within the TashAT gene cluster of the parasite genome. In this study, TashAT2 was transfected into bovine BoMac cells to generate three expressing and three non-expressing (opposite orientation) cell lines. RNA-Seq was conducted and differentially expressed (DE) genes identified. The resulting dataset was compared with genes differentially expressed between infected cells and non-infected cells, and DE genes between infected cell lines from susceptible Holstein vs tolerant Sahiwal cattle. Over 800 bovine genes displayed differential expression associated with TashAT2, 209 of which were also modulated by parasite infection. Network analysis showed enrichment of DE genes in pathways associated with cellular adhesion, oncogenesis and developmental regulation by mammalian AT-hook bearing high mobility group A (HMGA) proteins. Overlap of TashAT2 DE genes with Sahiwal vs Holstein DE genes revealed that a significant number of shared genes were associated with disease susceptibility. Altered protein levels encoded by one of these genes (GULP1) was strongly linked to expression of TashAT2 in BoMac cells and was demonstrated to be higher in infected Holstein leucocytes compared to Sahiwal. We conclude that TashAT2 operates as an HMGA analogue to differentially mould the epigenome of the infected cell and influence disease susceptibility.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Shiels, Professor Brian and Larcombe, Dr Stephen and Weir, Professor Willie and Capewell, Dr Paul and Kinnaird, Dr Jane |
| Creator Roles: | Kinnaird, J.Formal analysis, Investigation, Validation, Writing – review and editing Capewell, P.Investigation, Methodology, Writing – review and editing Larcombe, S.Formal analysis, Investigation, Writing – review and editing Weir, W.Formal analysis, Writing – review and editing Shiels, B.Conceptualization, Funding acquisition, Investigation, Project administration, Validation, Visualization, Writing – original draft, Writing – review and editing |
| Authors: | Durrani, Z., Kinnaird, J., Cheng, C. W., Brühlmann, F., Capewell, P., Jackson, A., Larcombe, S., Olias, P., Weir, W., and Shiels, B. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | PLoS ONE |
| Publisher: | Public Library of Science |
| ISSN: | 1932-6203 |
| ISSN (Online): | 1932-6203 |
| Copyright Holders: | Copyright © 2023 Durrani et al. |
| First Published: | First published in PLoS ONE 18(6): e0286526 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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