Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana

Kaba, G., Stevenson, A., Sakyi, S. A., Konney, T. O., Bhatia, R., Titiloye, N. A., Oppong, S. A., Agyemang-Yeboah, F., Cuschieri, K. and Graham, S. V. (2023) Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana. Tumour Virus Research, 15, 200261. (doi: 10.1016/j.tvr.2023.200261) (PMID:37179021) (PMCID:PMC10209332)

Abstract

Human papillomavirus (HPV) E6 and E7 oncogene expression is essential for cervical carcinogenesis. Evidence exists that E6/E7 variants may have different transforming activities while the risk of HPV-16 variants(A/D) differs by race/ethnicity. We determined the type-specific diversity of HPV infection in women with high grade cervical disease or cervical cancer in Ghana and investigated naturally occurring E6/E7 DNA variants in this population. HPV genotyping was carried out on 207 cervical swab samples collected from women referred to a gynaecology clinic at two teaching hospitals in Ghana. HPV-16, HPV-18 and HPV-45 were detected in 41.9%, 23.3% and 16.3% of cases respectively. HPV-16 E6/E7 DNA sequencing was performed in 36 samples. Thirty samples contained E6/E7 variants of the HPV-16-B/C lineage. 21/36 samples were of the HPV-16C1 sublineage variant and all contained the E7 A647G(N29S) single nucleotide polymorphism (SNP). This study reveals the diversity of E6/E7 DNA and the dominance of HPV16 B/C variants in cervicovaginal HPV infection in Ghana. Type-specific HPV diversity analysis indicates that most Ghanaian cervical disease cases are vaccine preventable. The study provides an important baseline from which for the impact of vaccine and antivirals on clinically relevant HPV infection and associated disease can be measured.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Stevenson, Mr Andrew and Graham, Professor Sheila
• Authors: Kaba, G., Stevenson, A., Sakyi, S. A., Konney, T. O., Bhatia, R., Titiloye, N. A., Oppong, S. A., Agyemang-Yeboah, F., Cuschieri, K., and Graham, S. V.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Tumour Virus Research
• Publisher: Elsevier
• ISSN: 2666-6790
• ISSN (Online): 2666-6790
• Published Online: 11 May 2023
• Copyright Holders: Copyright © 2023 The Authors
• First Published: First published in Tumour Virus Research 15: 200261
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
315250		MRC PE seed funding - John McLauchlan	John McLauchlan	Medical Research Council (MRC)	MC_UU_12014	III - Centre for Virus Research