Random access parallel microscopy

Ashraf, M., Mohanan, S. , Sim, B. R., Tam, A., Rahemipour, K., Brousseau, D., Thibault, S., Corbett, A. D. and Bub, G. (2021) Random access parallel microscopy. eLife, 10, e56426. (doi: 10.7554/eLife.56426) (PMID:33432922) (PMCID:PMC7843131)

Abstract

We introduce a random-access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long-duration records from different samples in parallel, which is not possible using conventional automated microscopes. The system is demonstrated by continuously imaging multiple cardiac monolayer and Caenorhabditis elegans preparations.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mohanan, Ms Sharika

Creator Roles

Mohanan, S.: Software, Formal analysis, Investigation

• Authors: Ashraf, M., Mohanan, S., Sim, B. R., Tam, A., Rahemipour, K., Brousseau, D., Thibault, S., Corbett, A. D., and Bub, G.
• College/School: College of Science and Engineering > School of Physics and Astronomy
• Journal Name: eLife
• Publisher: eLife Sciences Publications
• ISSN: 2050-084X
• ISSN (Online): 2050-084X
• Published Online: 12 January 2021
• Copyright Holders: Copyright © 2021 Ashraf et al.
• First Published: First published in eLife 10: e56426
• Publisher Policy: Reproduced under a Creative Commons License