A urinary peptidomics approach for early stages of cardiovascular disease risk: the African-PREDICT study

de Beer, D., Mels, C. M. C., Schutte, A. E., Delles, C. , Mary, S. , Mullen, W. , Mischak, H. and Kruger, R. (2023) A urinary peptidomics approach for early stages of cardiovascular disease risk: the African-PREDICT study. Hypertension Research, 46(2), pp. 485-494. (doi: 10.1038/s41440-022-01097-7) (PMID:36396816)

Abstract

Cardiovascular disease (CVD) affects individuals across the lifespan, with multiple cardiovascular (CV) risk factors increasingly present in young populations. The underlying mechanisms in early cardiovascular disease development are complex and still poorly understood. We therefore employed urinary proteomics as a novel approach to gain better insight into early CVD-related molecular pathways based on a CVD risk stratification approach. This study included 964 apparently healthy (no self-reported chronic illnesses, free from clinical symptoms of CVD) black and white men and women (aged 20–30 years old) from the African Prospective study on the Early Detection and Identification of Cardiovascular disease and Hypertension (African-PREDICT) study. Cardiovascular risk factors used for stratification included obesity, physical inactivity, tobacco use, high alcohol intake, hyperglycemia, dyslipidemia and hypertension. Participants were divided into low (0 risk factors), medium (1–2 risk factors) and high (≥3 risk factors) CV risk groups. We analyzed urinary peptidomics by capillary electrophoresis time-of-flight mass spectrometry. After adjusting for ethnicity, sex and age, 65 sequenced urinary peptides were differentially expressed between the CV risk groups (all q-values ≤ 0.01). These peptides included a lower abundance of collagen type I- and III-derived peptides in the high compared to the low CV risk group. With regard to noncollagen peptides, we found a lower abundance of alpha-1-antitrypsin fragments in the high compared to the low CV risk group (all q-values ≤ 0.01). Our findings indicate lower abundances of collagen types I and III in the high compared to the low CV risk group, suggesting potential early alterations in the CV extracellular matrix.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mischak, Professor Harald and Mullen, Dr Bill and Samji, Dr Sheon and Delles, Professor Christian
• Authors: de Beer, D., Mels, C. M. C., Schutte, A. E., Delles, C., Mary, S., Mullen, W., Mischak, H., and Kruger, R.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Hypertension Research
• Publisher: Springer Nature
• ISSN: 0916-9636
• ISSN (Online): 1348-4214
• Published Online: 17 November 2022
• Copyright Holders: Copyright © 2022 The Authors, under exclusive licence to The Japanese Society of Hypertension
• First Published: First published in Hypertension Research 46(2):485-494
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher