Mohanan, S. and Corbett, A. D. (2023) Understanding the limits of remote focusing. Optics Express, 31(10), pp. 16281-16294. (doi: 10.1364/OE.485635) (PMID:37157710)
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Abstract
It has previously been demonstrated in both simulation and experiment that well aligned remote focusing microscopes exhibit residual spherical aberration outside the focal plane. In this work, compensation of the residual spherical aberration is provided by the correction collar on the primary objective, controlled by a high precision stepper motor. A Shack-Hartmann wave front sensor is used to demonstrate the magnitude of the spherical aberration generated by the correction collar matches that predicted by an optical model of the objective lens. The limited impact of spherical aberration compensation on the diffraction limited range of the remote focusing system is described through a consideration of both on-axis and off-axis comatic and astigmatic aberrations, which are an inherent feature of remote focusing microscopes.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Mohanan, Ms Sharika |
| Authors: | Mohanan, S., and Corbett, A. D. |
| College/School: | College of Science and Engineering > School of Physics and Astronomy |
| Journal Name: | Optics Express |
| Publisher: | Optica Publishing Group |
| ISSN: | 1094-4087 |
| ISSN (Online): | 1094-4087 |
| Published Online: | 01 May 2023 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Optics Express 31(10): 16281-16294 |
| Publisher Policy: | Reproduced under a Creative Commons License |
| Data DOI: | 10.24378/exe.4605 |
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