Fine-tuning regulation of surface mobility by acrylate copolymers and its effect on cell adhesion and differentiation

Morata-Martínez, M., Sprott, M. R. , Antolinos-Turpín, C. M., Salmeron-Sanchez, M. and Gallego-Ferrer, G. (2023) Fine-tuning regulation of surface mobility by acrylate copolymers and its effect on cell adhesion and differentiation. ACS Applied Bio Materials, 6(5), pp. 1755-1762. (doi: 10.1021/acsabm.2c01053) (PMID:37067245) (PMCID:PMC10189726)

Abstract

Fibronectin (FN) mediates cell-material interactions during events such as tissue repair, and therefore the biomimetic modeling of this protein in vitro benefits regeneration. The nature of the interface is crucial in determining cell adhesion, morphology, and differentiation. Poly(ethyl acrylate) (PEA) spontaneously organizes FN into biological nanonetworks, resulting in exceptional bone regeneration in animal models. Spontaneous network organization of FN is also observed in poly(buthyl acrylate) (PBA) substrates that have higher surface mobility than PEA. C2C12 myoblasts differentiate efficiently on PEA and PBA substrates. In this study, we investigate if intermediate surface mobilities between PEA and PBA induce cell differentiation more efficiently than PEA. A family of P(EA-co-BA) copolymers were synthesized in the entire range of compositions to finely tune surface mobility between PEA and PBA. Surface characterization demonstrates that FN mobility steadily increased with the PBA content. All compositions allowed the biological organization of FN with similar exposure of cell binding domains. C2C12 myoblasts adhered well in all the materials, with higher focal adhesions in PEA and PBA. The increase of the interfacial mobility had an impact in cell adhesion by increasing the number of FAs per cell. In addition, cell differentiation decreased proportionally with surface mobility, from PEA to PBA.

• Item Type: Articles
• Additional Information: This work was supported by an EPSRC Programme Grant (EP/P001114/1), the Grant (PID2019−106000RB-C21) funded by MCIN/AEI/10.13039/501100011033 and the CIBER (Consorcio Centro de Investigacioń Biomedica ́ en Red) (CB06/01/1026), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacion.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Sprott, Dr Mark and Morata Martinez, Miranda
• Authors: Morata-Martínez, M., Sprott, M. R., Antolinos-Turpín, C. M., Salmeron-Sanchez, M., and Gallego-Ferrer, G.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Applied Bio Materials
• Publisher: American Chemical Society (ACS)
• ISSN: 2576-6422
• ISSN (Online): 2576-6422
• Published Online: 17 April 2023
• Copyright Holders: Copyright © 2023 The Authors
• First Published: First published in ACS Applied Bio Materials 6(5): 1755–1762
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
173192		Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicine	Manuel Salmeron-Sanchez	Engineering and Physical Sciences Research Council (EPSRC)	EP/P001114/1	ENG - Biomedical Engineering