Single-shot time-folded fluorescence lifetime imaging

Kapitany, V., Zickus, V., Fatima, A., Carles, G. and Faccio, D. (2023) Single-shot time-folded fluorescence lifetime imaging. Proceedings of the National Academy of Sciences of the United States of America, 120(16), e2214617120. (doi: 10.1073/pnas.2214617120) (PMID:37043531) (PMCID:PMC10120087)

Abstract

Fluorescence lifetime imaging is an important tool in bioimaging that allows one to detect subtle changes in cell dynamics and their environment. Most time-domain approaches currently involve scanning a single illumination point across the sample, which can make imaging dynamic scenes challenging, while single-shot “rapid lifetime determination” can suffer from large uncertainties when the lifetime is not appropriately sampled. Here, we propose a time-folded fluorescence lifetime imaging microscopy (TFFLIM) approach, whereby a time-folding cavity provides multiple spatially sheared replicas of the lifetime, each shifted temporally with respect to a fixed time gate. This provides a robust, single-shot FLIM approach that we experimentally validate across a broad lifetime range on fluorescent beads and Convallaria samples.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Zickus, Dr Vytautas and Carles Santacana, Dr Guillem and Kapitany, Mr Valentin and Faccio, Professor Daniele and Fatima, Dr Areeba
• Authors: Kapitany, V., Zickus, V., Fatima, A., Carles, G., and Faccio, D.
• College/School: College of Science and Engineering > School of Physics and Astronomy
• Journal Name: Proceedings of the National Academy of Sciences of the United States of America
• Publisher: National Academy of Sciences
• ISSN: 0027-8424
• ISSN (Online): 1091-6490
• Published Online: 12 April 2023
• Copyright Holders: Copyright © 2023 The Authors
• First Published: First published in Proceedings of the National Academy of Sciences of the United States of America 120(16): e2214617120
• Publisher Policy: Reproduced under a Creative Commons License
• Data DOI: 10.5525/gla.researchdata.1380

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
306629		Harnessing the physics of light to reveal mechanisms of multicellular shape and movement	Laura Machesky	Engineering and Physical Sciences Research Council (EPSRC)	EP/T002123/1	CS - Beatson Institute for Cancer Research
305153		RAEng Chair Emerging Technologies	Daniele Faccio	Royal Academy of Engineering (RAE)	CiET1819/20	P&S - Physics & Astronomy