Single-shot time-folded fluorescence lifetime imaging

Kapitany, V., Zickus, V., Fatima, A., Carles, G. and Faccio, D. (2023) Single-shot time-folded fluorescence lifetime imaging. Proceedings of the National Academy of Sciences of the United States of America, 120(16), e2214617120. (doi: 10.1073/pnas.2214617120) (PMID:37043531) (PMCID:PMC10120087)

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Fluorescence lifetime imaging is an important tool in bioimaging that allows one to detect subtle changes in cell dynamics and their environment. Most time-domain approaches currently involve scanning a single illumination point across the sample, which can make imaging dynamic scenes challenging, while single-shot “rapid lifetime determination” can suffer from large uncertainties when the lifetime is not appropriately sampled. Here, we propose a time-folded fluorescence lifetime imaging microscopy (TFFLIM) approach, whereby a time-folding cavity provides multiple spatially sheared replicas of the lifetime, each shifted temporally with respect to a fixed time gate. This provides a robust, single-shot FLIM approach that we experimentally validate across a broad lifetime range on fluorescent beads and Convallaria samples.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Zickus, Dr Vytautas and Carles Santacana, Dr Guillem and Kapitany, Mr Valentin and Faccio, Professor Daniele and Fatima, Dr Areeba
Authors: Kapitany, V., Zickus, V., Fatima, A., Carles, G., and Faccio, D.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Proceedings of the National Academy of Sciences of the United States of America
Publisher:National Academy of Sciences
ISSN (Online):1091-6490
Published Online:12 April 2023
Copyright Holders:Copyright © 2023 The Authors
First Published:First published in Proceedings of the National Academy of Sciences of the United States of America 120(16): e2214617120
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.1380

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
306629Harnessing the physics of light to reveal mechanisms of multicellular shape and movementLaura MacheskyEngineering and Physical Sciences Research Council (EPSRC)EP/T002123/1CS - Beatson Institute for Cancer Research
305153RAEng Chair Emerging TechnologiesDaniele FaccioRoyal Academy of Engineering (RAE)CiET1819/20P&S - Physics & Astronomy