Muñoz-Prieto, A., Contreras-Aguilar, M. D., Cerón, J. J., Ayala de la Peña, I., Martín-Cuervo, M., Eckersall, P. D. , Holm Henriksen, I.-M., Tecles, F. and Hansen, S. (2023) Changes in calprotectin (S100A8-A9) and aldolase in the saliva of horses with equine gastric ulcer syndrome. Animals, 13(8), 1367. (doi: 10.3390/ani13081367) (PMID:37106929) (PMCID:PMC10135270)
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Abstract
Equine gastric ulcer syndrome (EGUS) is a highly prevalent disease that affects horses worldwide. Within EGUS, two different forms have been described: equine squamous gastric disease (ESGD) and equine glandular gastric disease (EGGD). The associated clinical signs cause detrimental activity performance, reducing the quality of life of animals. Saliva can contain biomarkers for EGUS that could be potentially used as a complementary tool for diagnosis. The objective of this work was to evaluate the measurements of calprotectin (CALP) and aldolase in the saliva of horses as potential biomarkers of EGUS. For this purpose, automated assays for the quantification of these two proteins were analytically validated and applied for detecting EGUS in a total of 131 horses divided into 5 groups: healthy horses, ESGD, EGGD, combined ESGD and EGGD, and horses with other intestinal pathologies. The assays showed good precision and accuracy in analytical validation, and they were able to discriminate between horses with EGUS and healthy horses, especially in the case of CALP, although they did not show significant differences between horses with EGUS and horses with other diseases. In conclusion, salivary CALP and aldolase can be determined in the saliva of horses and further studies are warranted to elucidate the potential of these analytes as biomarkers in EGUS.
Item Type: | Articles |
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Additional Information: | This research was funded by the Seneca foundation of Murcia Regional Government, Spain, grant number 19894/GERM/15. A.M.-P. was funded by a post-doctoral fellowship “Ramón y Cajal” supported by the Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación (AEI), Spain, and The European Next Generation Funds (NextgenerationEU) (RYC2021-033660-I). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Eckersall, Professor David |
Authors: | Muñoz-Prieto, A., Contreras-Aguilar, M. D., Cerón, J. J., Ayala de la Peña, I., Martín-Cuervo, M., Eckersall, P. D., Holm Henriksen, I.-M., Tecles, F., and Hansen, S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Animals |
Publisher: | MDPI |
ISSN: | 2076-2615 |
ISSN (Online): | 2076-2615 |
Published Online: | 16 April 2023 |
Copyright Holders: | Copyright © 2023 The Authors |
First Published: | First published in Animals 13(8): 1367 |
Publisher Policy: | Reproduced under a Creative Commons License |
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