Development of Acanthocheilonema viteae in Meriones shawi : Absence of microfilariae and production of active ES‐62

Lumb, F. E., Doonan, J. , Corbet, M., Pineda, M. A. , Harnett, M. M. and Harnett, W. (2021) Development of Acanthocheilonema viteae in Meriones shawi : Absence of microfilariae and production of active ES‐62. Parasite Immunology, 43(3), e12803. (doi: 10.1111/pim.12803) (PMID:33091157) (PMCID:PMC7988569)

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Aims ES-62 is a well-studied anti-inflammatory molecule secreted by L4-adult stage Acanthocheilonema viteae. We maintain the life cycle of A viteae using Meriones libycus as the definitive host. Here, we investigated whether the full life cycle could be maintained, and functional ES-62 produced, in a related jird species—Meriones shawi. Methods and Results Adult worms were produced in comparable numbers in the two species, but very few microfilariae (MF) were observed in the M shawi bloodstream. M shawi ES-62 produced ex vivo was functional and protective in a mouse model of arthritis. Myeloid-derived cells from naïve and infected jirds of both species were compared with respect to ROS production and osteoclast generation, and some differences between the two species in both the absence and presence of infection were observed. Conclusions The life cycle of A viteae cannot be successfully completed in M shawi jirds but L3 stage worms develop to adulthood and produce functional ES-62. Preliminary investigation into jird immune responses suggests that infection can differentially modulate myeloid responses in the two species. However, species-specific reagents are required to understand the complex interplay between A viteae and its host and to explain the lack of circulating MF in infected M shawi jirds.

Item Type:Articles
Additional Information:Funding information: This work was funded by awards to WH and MMH from Arthritis Research UK (21133) and the BBSRC (BB/M029662/1 and BB/M029727/1).
Glasgow Author(s) Enlighten ID:Corbet, Marlene and Harnett, Professor Margaret and Pineda, Dr Miguel and Doonan, Dr James
Authors: Lumb, F. E., Doonan, J., Corbet, M., Pineda, M. A., Harnett, M. M., and Harnett, W.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Parasite Immunology
ISSN (Online):1365-3024
Published Online:22 October 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Parasite Immunology 43(3):e12803
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172155MIMIC - Do parasitic worms and their secreted immunomodulators protect against musculosketal disease by impacting on the host microbiome?Margaret HarnettVersus Arthritis (ARTRESUK)21133III - Immunology
172179Can studying the mechanism of action of the parasitic worm-derived immunomodulator ES-62, inform on how to slow ageing and improve healthspan?Margaret HarnettBiotechnology and Biological Sciences Research Council (BBSRC)BB/M029727/1III - Immunology