Alexander, A. J.T. et al. (2023) Characterisation of the antiviral RNA interference response to Toscana virus in sand fly cells. PLoS Pathogens, 19(3), e1011283. (doi: 10.1371/journal.ppat.1011283) (PMID:36996243) (PMCID:PMC10112792)
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Abstract
Toscana virus (TOSV) (Bunyavirales, Phenuiviridae, Phlebovirus, Toscana phlebovirus) and other related human pathogenic arboviruses are transmitted by phlebotomine sand flies. TOSV has been reported in nations bordering the Mediterranean Sea among other regions. Infection can result in febrile illness as well as meningitis and encephalitis. Understanding vector-arbovirus interactions is crucial to improving our knowledge of how arboviruses spread, and in this context, immune responses that control viral replication play a significant role. Extensive research has been conducted on mosquito vector immunity against arboviruses, with RNA interference (RNAi) and specifically the exogenous siRNA (exo-siRNA) pathway playing a critical role. However, the antiviral immunity of phlebotomine sand flies is less well understood. Here we were able to show that the exo-siRNA pathway is active in a Phlebotomus papatasi-derived cell line. Following TOSV infection, distinctive 21 nucleotide virus-derived small interfering RNAs (vsiRNAs) were detected. We also identified the exo-siRNA effector Ago2 in this cell line, and silencing its expression rendered the exo-siRNA pathway largely inactive. Thus, our data show that this pathway is active as an antiviral response against a sand fly transmitted bunyavirus, TOSV.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Varjak, Dr Margus and Ratinier, Dr Maxime and Alexander, Dr Akira and Vattipally, Dr Sreenu and Brennan, Dr Benjamin and Hughes, Dr Joseph and Arnaud, Mr Frederick and Kohl, Professor Alain |
| Authors: | Alexander, A. J.T., Salvemini, M., Sreenu, V. B., Hughes, J., Telleria, E. L., Ratinier, M., Arnaud, F., Volf, P., Brennan, B., Varjak, M., and Kohl, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | PLoS Pathogens |
| Publisher: | Public Library of Science |
| ISSN: | 1553-7366 |
| ISSN (Online): | 1553-7374 |
| Published Online: | 30 March 2023 |
| Copyright Holders: | Copyright © 2023 Alexander et al. |
| First Published: | First published in PLoS Pathogens 19(3): e1011283 |
| Publisher Policy: | Reproduced under a Creative Commons License |
| Data DOI: | 10.5525/gla.researchdata.1373 |
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