Arylimidamides have potential for chemoprophylaxis against blood-transmitted Chagas disease

Timm, B. L., da Gama, A. N. S., Batista, M. M., Batista, D. d. G. J., Boykin, D. W., De Koning, H. P. and Correia Soeiro, M. d. N. (2023) Arylimidamides have potential for chemoprophylaxis against blood-transmitted Chagas disease. Pathogens, 12(5), 701. (doi: 10.3390/pathogens12050701) (PMID:37242371) (PMCID:PMC10224031)

Abstract

Chagas disease (CD) affects over 6 million people worldwide and can be transmitted iatrogenically. Crystal violet (CV) was previously used for pathogen reduction but has harmful side-effects. In the present study, three arylimidamides (AIAs) and CV were used to sterilize mice blood samples experimentally contaminated with bloodstream trypomastigotes (BT) of Trypanosoma cruzi, at non hemolytic doses. All AIAs were not toxic to mouse blood cells until the highest tested concentration (96 µM). The previous treatment of BT with the AIAs impaired the infection establishment of cardiac cell cultures. In vivo assays showed that pre-incubation of mouse blood samples with the AIAs and CV (96 µM) significantly suppressed the parasitemia peak, but only the AIA DB1831 gave ≥90% animal survival, while vehicle treated samples reached 0%. Our findings support further studies regarding the potential use of AIAs for blood bank purposes.

• Item Type: Articles
• Additional Information: This research was funded by Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Oswaldo Cruz, PAEF/CNPq/Fiocruz, PDTIS, and CAPES. M.N.C. is research fellow from CNPq and CNE/FAPERJ.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: De Koning, Professor Harry
• Authors: Timm, B. L., da Gama, A. N. S., Batista, M. M., Batista, D. d. G. J., Boykin, D. W., De Koning, H. P., and Correia Soeiro, M. d. N.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Pathogens
• Publisher: MDPI
• ISSN: 2076-0817
• ISSN (Online): 2076-0817
• Published Online: 12 May 2023
• Copyright Holders: Copyright © 2023 The Authors
• First Published: First published in Pathogens 12(5): 701
• Publisher Policy: Reproduced under a Creative Commons License