Dong, J., Zeng, Z., Huang, Y., Chen, C., Cheng, Z. and Zhu, Q. (2023) Challenges and opportunities for circRNA identification and delivery. Critical Reviews in Biochemistry and Molecular Biology, 58(1), pp. 19-35. (doi: 10.1080/10409238.2023.2185764) (PMID:36916323)
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Abstract
Circular RNAs (circRNAs) are evolutionarily conserved noncoding RNAs with tissue-specific expression patterns, and exert unique cellular functions that have the potential to become biomarkers in therapeutic applications. Therefore, accurate and sensitive detection of circRNA with facile platforms is essential for better understanding of circRNA biological processes and circRNA-related disease diagnosis and prognosis; and precise regulation of circRNA through efficient delivery of circRNA or siRNA is critical for therapeutic purposes. Here, we reviewed the current development of circRNA identification methodologies, including overviewing the purification steps, summarizing the sequencing methods of circRNA, as well as comparing the advantages and disadvantages of traditional and new detection methods. Then, we discussed the delivery and manipulation strategies for circRNAs in both research and clinic treatment. Finally, the challenges and opportunities of analyzing circRNAs were addressed.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by the Hunan Provincial Natural Science Foundation of China (2021JJ30916) (2021JJ80078) (2022JJ10091) and National Natural Science Foundation of China (32270609). |
| Keywords: | Circular RNA, analysis, identification, detection, biosensors. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Zeng, Zhuoer |
| Authors: | Dong, J., Zeng, Z., Huang, Y., Chen, C., Cheng, Z., and Zhu, Q. |
| College/School: | College of Science and Engineering > School of Engineering |
| Journal Name: | Critical Reviews in Biochemistry and Molecular Biology |
| Publisher: | Taylor & Francis |
| ISSN: | 1040-9238 |
| ISSN (Online): | 1549-7798 |
| Published Online: | 14 March 2023 |
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