New technologies to study helminth development and host-parasite interactions

Britton, C. , Laing, R. , McNeilly, T. N., Perez, M. G. , Otto, T. D. , Hildersley, K. A., Maizels, R. M. , Devaney, E. and Gillan, V. (2023) New technologies to study helminth development and host-parasite interactions. International Journal for Parasitology, 53(8), pp. 393-403. (doi: 10.1016/j.ijpara.2022.11.012) (PMID:36931423)

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How parasites develop and survive, and how they stimulate or modulate host immune responses are important in understanding disease pathology and for the design of new control strategies. Microarray analysis and bulk RNA sequencing have provided a wealth of data on gene expression as parasites develop through different life-cycle stages and on host cell responses to infection. These techniques have enabled gene expression in the whole organism or host tissue to be detailed, but do not take account of the heterogeneity between cells of different types or developmental stages, nor the spatial organisation of these cells. Single-cell RNA-seq (scRNA-seq) adds a new dimension to studying parasite biology and host immunity by enabling gene profiling at the individual cell level. Here we review the application of scRNA-seq to establish gene expression cell atlases for multicellular helminths and to explore the expansion and molecular profile of individual host cell types involved in parasite immunity and tissue repair. Studying host-parasite interactions in vivo is challenging and we conclude this review by briefly discussing the applications of organoids (stem-cell derived mini-tissues) to examine host-parasite interactions at the local level, and as a potential system to study parasite development in vitro. Organoid technology and its applications have developed rapidly, and the elegant studies performed to date support the use of organoids as an alternative in vitro system for research on helminth parasites.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen and Mcneilly, Dr Tom and Britton, Professor Collette and Otto, Professor Thomas and Laing, Dr Roz and Gillan, Dr Victoria and Hildersley, Katie and Perez, Dr Matias and Maizels, Professor Rick
Authors: Britton, C., Laing, R., McNeilly, T. N., Perez, M. G., Otto, T. D., Hildersley, K. A., Maizels, R. M., Devaney, E., and Gillan, V.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Research Centre:College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology
Journal Name:International Journal for Parasitology
Publisher:Elsevier for Australian Society for Parasitology
ISSN (Online):1879-0135
Published Online:16 March 2023
Copyright Holders:Copyright © 2023 The Authors
First Published:First published in International Journal for Parasitology 53(8):393-403
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
302416Tuft cells, sensors adn effectors in immunity to helminthsRichard MaizelsWellcome Trust (WELLCOTR)211814/Z/18/ZIII - Parasitology
306505Hitting a moving target: genomic and metabolic approaches to improve anthelmintic use in evolving helminth populationsRosalind LaingWellcome Trust (WELLCOTR)216614/Z/19/ZMVLS - Polyomics Facility
310820Effects of parasitic helminth secreted microRNAs on host gastrointestinal epithelial cellsCollette BrittonThe Royal Society (ROYSOC)NIF\R1\201159Institute of Biodiversity, Animal Health and Comparative Medicine
170547The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/ZIII - Parasitology
308411Molecular and Cellular Interactions in Helminth InfectionsRichard MaizelsWellcome Trust (WELLCOTR)219530/Z/19/ZIII - Parasitology