A palette of minimally tagged sucrose analogues for real‐time Raman imaging of intracellular plant metabolism

Moliner, F., Knox, K. , Gordon, D., Lee, M., Tipping, W. J., Geddis, A., Reinders, A., Ward, J. M., Oparka, K. and Vendrell, M. (2021) A palette of minimally tagged sucrose analogues for real‐time Raman imaging of intracellular plant metabolism. Angewandte Chemie (International Edition), 60(14), pp. 7637-7642. (doi: 10.1002/anie.202016802) (PMID:33491852) (PMCID:PMC8048481)

Abstract

Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Knox, Dr Kirsten
• Authors: Moliner, F., Knox, K., Gordon, D., Lee, M., Tipping, W. J., Geddis, A., Reinders, A., Ward, J. M., Oparka, K., and Vendrell, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Angewandte Chemie (International Edition)
• Publisher: Wiley
• ISSN: 1433-7851
• ISSN (Online): 1521-3773
• Published Online: 25 January 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Angewandte Chemie (International Edition) 60(14): 7637-7642
• Publisher Policy: Reproduced under a Creative Commons License