Peinado-Izaguerri, J. , Zarzuela, E., McLaughlin, M. , Small, A. C., Riva, F., McKeegan, D. E.F. , Bain, M. , Muñoz, J., Bhide, M. and Preston, T. (2023) A novel dynamic proteomics approach for the measurement of broiler chicken protein fractional synthesis rate. Rapid Communications in Mass Spectrometry, 37(10), e9497. (doi: 10.1002/rcm.9497) (PMID:36851885)
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Abstract
Rationale: The study of protein synthesis in farm animals is uncommon despite of its potential to increase knowledge about metabolism and discover new biomarkers of health and growth status. The present study describes a novel dynamic proteomics approach for the measurement of protein FSR in broiler chickens. Methods: Chickens received a 10g/kg oral dose of 2H2O at day 21 of their life. Body water 2H abundance was measured in plasma samples using a portable FTIR spectrometer. Free and protein-bound AAs were isolated and had their 2H enrichment measured by GC/MS. Peptide 2H enrichment was measured by proteomics analysis of plasma and muscle samples. Albumin, fibrinogen and muscle protein FSR were calculated from GC/MS and proteomics data. Results: Ala appeared to be more enriched at the site of protein synthesis than in the AA free pools. Glu was found to be the AA closest to isotopic equilibrium between the different AA pools. Glu was used as an anchor to calculate n (AA) values necessary for chicken protein FSR calculation in dynamic proteomics studies. FSR values calculated using proteomics data and GC/MS data showed good agreement as evidenced by a Bland-Altman residual plot. Conclusions: A new dynamic proteomics approach for the measurement of broiler chicken individual protein FSR based on the administration of a single 2H2O oral bolus has been developed and validated. The proposed approach could facilitate new immunological and nutritional studies on free living animals.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | McLaughlin, Dr Mark and McKeegan, Dr Dorothy and Preston, Professor Tom and Riva, Francesca and Bain, Professor Maureen and Peinado, Mr Jorge |
| Authors: | Peinado-Izaguerri, J., Zarzuela, E., McLaughlin, M., Small, A. C., Riva, F., McKeegan, D. E.F., Bain, M., Muñoz, J., Bhide, M., and Preston, T. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine College of Science and Engineering > Scottish Universities Environmental Research Centre |
| Journal Name: | Rapid Communications in Mass Spectrometry |
| Publisher: | Wiley |
| ISSN: | 0951-4198 |
| ISSN (Online): | 1097-0231 |
| Published Online: | 27 February 2023 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Rapid Communications in Mass Spectrometry 37(10): e9497 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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